Horikoshi T, Fukuzawa K, Hanada N, Ezoe K, Eguchi H, Hamaoka S, Tsujiya H, Tsukamoto T
Department of Dermatology and Urology, School of Medicine, Sapporo Medical University, Japan.
J Dermatol. 1995 Sep;22(9):631-6. doi: 10.1111/j.1346-8138.1995.tb03889.x.
We have studied the effects of interferon (IFN)-alpha, beta, and gamma in vitro on the growth and invasive potential of human melanoma SK-MEL-118 cells. The antiproliferative effects of IFNs were assessed by a quantitative regrowth assay in which cells were treated with IFNs at concentrations of 10(2), 10(3) or 10(4) IU/ml for 3 days (until day 4) and then further incubated without IFNs for 7 days (until day 11). The growth inhibitory effect of each IFN on melanoma cells was dose- and time-dependent. Among these three types of IFNs, however, IFN-beta exerted the strongest inhibitory effect on cell growth. To assess the anti-invasive effect of each IFN on melanoma cells, we employed an in vitro assay system using matrigel-coated Transwell chambers. When cells were treated with 10(2), 10(3), or 10(4) IU/ml of the three types of IFNs for 24 hours, the amount of tritiated thymidine incorporated into melanoma cells were treated for 24 hours with 10(4) IU/ml of IFN-beta or gamma prior to the assay, the number of cells that invaded the filter decreased by 40%; this decrease was only 10% with the same amount of IFN-alpha. Simultaneous addition of IFNs during the invasion assay was not effective in any combination. Only when the cells were pretreated with IFNs, antiinvasive effects against melanoma cells were exerted. IFN-alpha was less inhibitory than IFN-beta or gamma on proliferation and not at all inhibitory on invasion. Considering both the antiproliferative and antiinvasive effects of IFNs, our results suggest that IFN-beta has the strongest antitumoral effect on human melanoma cells.(ABSTRACT TRUNCATED AT 250 WORDS)
我们在体外研究了α、β和γ干扰素对人黑色素瘤SK-MEL-118细胞生长和侵袭潜能的影响。通过定量再生长试验评估干扰素的抗增殖作用，即细胞分别用浓度为10²、10³或10⁴ IU/ml的干扰素处理3天（至第4天），然后在无干扰素的条件下继续培养7天（至第11天）。每种干扰素对黑色素瘤细胞的生长抑制作用呈剂量和时间依赖性。然而，在这三种类型的干扰素中，β干扰素对细胞生长的抑制作用最强。为了评估每种干扰素对黑色素瘤细胞的抗侵袭作用，我们采用了一种使用基质胶包被的Transwell小室的体外检测系统。当细胞用三种类型的干扰素（10²、10³或10⁴ IU/ml）处理24小时后，在检测前用10⁴ IU/ml的β或γ干扰素处理黑色素瘤细胞24小时，侵袭滤膜的细胞数量减少了40%；而相同剂量的α干扰素处理时，细胞数量仅减少10%。在侵袭试验中同时添加干扰素，无论何种组合均无效。只有当细胞用干扰素预处理时，才会对黑色素瘤细胞产生抗侵袭作用。α干扰素在增殖方面的抑制作用比β或γ干扰素弱，且对侵袭完全没有抑制作用。综合考虑干扰素的抗增殖和抗侵袭作用，我们的结果表明β干扰素对人黑色素瘤细胞具有最强的抗肿瘤作用。（摘要截断于250字）
