Widyowati Retno, Sugimoto Sachiko, Yamano Yoshi, Otsuka Hideaki, Matsunami Katsuyoshi
Graduate School of Biomedical & Health Sciences, Hiroshima University.
Chem Pharm Bull (Tokyo). 2016;64(5):517-21. doi: 10.1248/cpb.c16-00073.
Three new flavonoid glycosides named isolinariins C, D and E (1-3), two known flavonoid glycosides (4, 5) and three known flavonoids (6-8) were isolated from the whole plant of Linaria japonica. The structures of these compounds were determined mainly by spectroscopic analyses. The bioactivities of these isolated compounds were evaluated for their inhibitory activities against human cell line A549, collagenase, and advanced glycation end product (AGE) formation. Among the isolated compounds, isolinariins C, D and E (1, 2 and 3) showed inhibition toward AGE formation (IC50 values of 34.8, 35.0 and 19.5 µM, respectively). And linariin (4), pectolinarin (5) and luteolin (8) were found to be active against collagenase with IC50 values of 79.4, 78.6 and 40.5 µM, respectively, without significant cytotoxicity at these concentrations.
从日本柳穿鱼全草中分离出三种新的黄酮苷，分别命名为异柳穿鱼苷C、D和E（1-3），两种已知的黄酮苷（4, 5）和三种已知的黄酮类化合物（6-8）。这些化合物的结构主要通过光谱分析确定。对这些分离得到的化合物的生物活性进行了评估，检测了它们对人细胞系A549、胶原酶以及晚期糖基化终产物（AGE）形成的抑制活性。在分离得到的化合物中，异柳穿鱼苷C、D和E（1、2和3）对AGE形成具有抑制作用（IC50值分别为34.8、35.0和19.5 μM）。并且发现柳穿鱼苷（4）、果胶柳穿鱼苷（5）和木犀草素（8）对胶原酶有活性，IC50值分别为79.4、78.6和40.5 μM，在这些浓度下无明显细胞毒性。
