Zhang Shou-Chi, Lai Qi-Heng, Lu Yuan, Liu Zhi-Dan, Wang Tian-Min, Zhang Chong, Xing Xin-Hui
Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
J Biosci Bioeng. 2016 Oct;122(4):482-7. doi: 10.1016/j.jbiosc.2016.03.014. Epub 2016 Apr 14.
Hydrogen was produced from steam-exploded corn stover by using a combination of the cellulolytic bacterium Clostridium cellulolyticum and non-cellulolytic hydrogen-producing bacteria. The highest hydrogen yield of the co-culture system with C. cellulolyticum and Citrobacter amalonaticus reached 51.9 L H2/kg total solid (TS). The metabolites from the co-culture system were significantly different from those of the mono-culture systems. Formate, which inhibits the growth of C. cellulolyticum, could be consumed by the hydrogen-evolving bacteria, and transformed into hydrogen. Glucose and xylose were released from corn stover via hydrolysis by C. cellulolyticum and were quickly utilized in dark fermentation with the co-cultured hydrogen-producing bacteria. Because the hydrolysis of corn stover by C. cellulolyticum was much slower than the utilization of glucose and xylose by the hydrogen-evolving bacteria, the sugar concentrations were always maintained at low levels, which favored a high hydrogen molar yield.
通过使用纤维素分解细菌解纤维梭菌（Clostridium cellulolyticum）和非纤维素分解产氢细菌的组合，从蒸汽爆破玉米秸秆中产生氢气。解纤维梭菌和丙二酸柠檬酸杆菌（Citrobacter amalonaticus）共培养系统的最高产氢量达到51.9升氢气/千克总固体（TS）。共培养系统的代谢产物与单培养系统的代谢产物有显著差异。抑制解纤维梭菌生长的甲酸盐可被产氢细菌消耗，并转化为氢气。葡萄糖和木糖通过解纤维梭菌的水解作用从玉米秸秆中释放出来，并在与共培养的产氢细菌进行暗发酵时迅速被利用。由于解纤维梭菌对玉米秸秆的水解速度远慢于产氢细菌对葡萄糖和木糖的利用速度，糖浓度始终保持在较低水平，这有利于高氢气摩尔产率。
Zotero 插件