Opoku-Temeng Clement, Sintim Herman O
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Center for Drug Discovery, Purdue University, West Lafayette, IN 47907, USA.
Sci Rep. 2016 May 6;6:25445. doi: 10.1038/srep25445.
Cyclic di-AMP has emerged as an important signaling molecule that controls a myriad of functions, including cell wall homeostasis in different bacteria. Polyphenols display various biological activities and tea polyphenols in particular have been shown to possess among other properties antioxidant and antibacterial activities. Certain tea polyphenols, such as catechin and epigallocatechin gallate, have been used to augment the action of traditional antibiotics that target the cell wall. Considering the expanding role played by cyclic dinucleotides in bacteria, we investigated whether the action of polyphenols on bacteria could be due in part to modulation of c-di-AMP signaling. Out of 14 tested polyphenols, tannic acid (TA), theaflavin-3'-gallate (TF2B) and theaflavin-3,3'-digallate (TF3) exhibited inhibitory effects on B. subtilis c-di-AMP synthase, DisA. TF2B and TF3 specifically inhibited DisA but not YybT (a PDE) whilst TA was more promiscuous and inhibited both DisA and YybT.
环二腺苷酸已成为一种重要的信号分子，可控制众多功能，包括不同细菌中的细胞壁稳态。多酚具有多种生物活性，尤其是茶多酚已被证明具有抗氧化和抗菌活性等多种特性。某些茶多酚，如儿茶素和表没食子儿茶素没食子酸酯，已被用于增强靶向细胞壁的传统抗生素的作用。考虑到环二核苷酸在细菌中发挥的作用不断扩大，我们研究了多酚对细菌的作用是否部分归因于对环二腺苷酸信号传导的调节。在14种测试的多酚中，单宁酸（TA）、茶黄素-3'-没食子酸酯（TF2B）和茶黄素-3,3'-二没食子酸酯（TF3）对枯草芽孢杆菌环二腺苷酸合酶DisA表现出抑制作用。TF2B和TF3特异性抑制DisA，但不抑制YybT（一种磷酸二酯酶），而TA则更具通用性，同时抑制DisA和YybT。
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