在经丁硫氨酸亚砜胺（BSO）或二乙基亚砜（DEM）耗竭后细胞硫醇恢复期间，米索硝唑的放射增敏作用。

Radiosensitization by misonidazole during recovery of cellular thiols following depletion by BSO or DEM.

作者信息

McNally N J, Soranson J A

机构信息

Cancer Research Campaign Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, England.

出版信息

Int J Radiat Oncol Biol Phys. 1989 May;16(5):1331-4. doi: 10.1016/0360-3016(89)90309-x.

DOI:10.1016/0360-3016(89)90309-x

PMID:2715087

Abstract

V79 cells have been depleted of their endogenous thiols by treatment with 100 microM BSO for 16-18 hr, or 0.5 mM DEM for 1 hr. The recovery of cellular thiols after removal of the drugs was determined by h.p.l.c. or flow cytometry and the sensitizer enhancement ratio for 100 microM misonidazole was measured as a function of time after removal of the drugs. The SER of 1.2 for control (hypoxic) cells increased to 1.8 for BSO treated (hypoxic) cells and 2.2 for DEM treated ones, when thiol levels were below 10% of controls. The SER and thiol levels returned to control values within 5 hr of removing DEM. After BSO there was little change during the first 5 hr and then a gradual return to normal values by 24 hrs. The major fall in the SER after removal of the drugs occurred as the cellular thiols increased to 60% of control values.

摘要

通过用100微摩尔/升的丁硫氨酸亚砜胺（BSO）处理16 - 18小时或0.5毫摩尔/升的二乙基亚硝胺（DEM）处理1小时，V79细胞内源性硫醇已被耗尽。去除药物后细胞硫醇的恢复情况通过高效液相色谱法（h.p.l.c.）或流式细胞术测定，并且测定了100微摩尔/升米索硝唑的敏化剂增强率与去除药物后时间的函数关系。当硫醇水平低于对照的10%时，对照（缺氧）细胞的敏化剂增强率（SER）为1.2，经BSO处理（缺氧）的细胞增加到1.8，经DEM处理的细胞增加到2.2。去除DEM后5小时内，SER和硫醇水平恢复到对照值。去除BSO后，最初5小时内变化不大，然后到24小时逐渐恢复到正常值。去除药物后SER的主要下降发生在细胞硫醇增加到对照值的60%时。