[Antiapoptotic Effect of the Leukemia Associated Gene MLAA-34 in HeLa Cells].
作者信息
Zhang Peng-Yu, Zhao Xuan, Zhang Wen-Juan, Zhang Wang-Gang, Chen Yin-Xia
机构信息
Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China. E-mail:
Department of Histology & Embryology, Xi'an Medical College, Xi'an 710021, Shaanxi Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Apr;24(2):363-8. doi: 10.7534/j.issn.1009-2137.2016.02.011.
OBJECTIVE
To study the antiapoptotic effect of leukemia-associated gene MLAA-34 in HeLa cells.
METHODS
The MLAA-34 recombinant lentiviral expression vector was constructed, and the stably transfected HeLa cell line with high expression of MLAA-34 was set up; As(2)O(3) was used to induce apoptosis; the MTT assay, colony formation test and flow cytometry were used to detect the ability of cell proliferation, colong formation, apoptosis and cell cycle changes respectively.
RESULTS
After treatment with As(2)O(3), the survival rate of HeLa cells with MLAA-34 overexpression was significantly higher than that of the control cells, and the colony formation ability of MLAA-34 significantly increased, and the high expression of MLAA-34 gene significantly decreased the apoptosis rate of HeLa cells, and decreased the proportion of G(2)/M phase cells.
CONCLUSION
The leukemia-associated gene MLAA-34 has been comfirmed to show antiapoptotic effect in HeLa cells which are induced by As(2)O(3).
Zotero 插件