Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628CN Delft, The Netherlands.
Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, UK.
Bioresour Technol. 2016 Aug;214:338-347. doi: 10.1016/j.biortech.2016.04.120. Epub 2016 Apr 28.
Using a high-salinity-adapted inoculum and a moderate stepwise-increased organic loading rate (OLR), a stable digester performance was achieved in treating sludge from a brackish aquaculture recirculation system. The specific methane yield was distinctly enhanced, reaching 0.203LCH4/gCODadded, compared to literature values (0.140-0.154LCH4/gCODadded) from the salty sludges. OLR adjustment and the fecal substrate substantially influenced population changes in the digester. Within the bacterial subpopulations, the relative abundance of Bacillus and Bacteroides declined, accompanied by the increase of Clostridium and Trigonala over time. The results show Trigonala was derived from the substrate and accumulated inside the digester. The most abundant methanogen was Methanosarcina in the inoculum and the digestates. The Methanosarcina proliferation can be ascribed to its metabolic versatility, probably a feature of crucial importance for high-salinity environments. Other frequently observed methanogens were outcompeted. The population similarity at the genus level between inoculum and digestates declined during the initial stage and afterwards increased.
采用高盐度适应接种物和适度逐步增加的有机负荷率（OLR），成功实现了处理来自咸水养殖循环系统的污泥的稳定消化器性能。与文献中（0.140-0.154LCH4/gCODadded）的盐分污泥相比，特定甲烷产率明显提高，达到 0.203LCH4/gCODadded。OLR 调整和粪便基质极大地影响了消化器中的种群变化。在细菌亚群中，芽孢杆菌和拟杆菌的相对丰度下降，伴随着梭菌和 Trigonala 的增加。结果表明，Trigonala 来自于基质并在消化器内积累。接种物和消化物中最丰富的产甲烷菌是 Methanosarcina。Methanosarcina 的增殖可以归因于其代谢多功能性，这可能是高盐环境中至关重要的特征。其他经常观察到的产甲烷菌被竞争淘汰。在初始阶段和之后，接种物和消化物之间属水平的种群相似性下降。
