Nie Xiaojing, Wang Xiaoting, Lin Youdong, Yu Yinghao, Liu Wei, Xie Feilai, Wang Xuzhou, Tan Jianming, Huang Qiaojia
Clin Lab. 2016;62(3):409-16. doi: 10.7754/clin.lab.2015.150722.
Cyclin-dependent kinase inhibitor 1A (CDKN1A) and Cyclin D1 (CCND1) play essential roles in the regulation of cell cycle progression and are closely associated with human cancer. CDKN1A and CCND1 single nucleotide polymorphisms (SNPs) have been demonstrated to influence the prognosis in humans with different cancers. However, their roles in the prognosis of patients with resected gastric adenocarcinoma (RGA) remain to be determined.
Genotypes of CDKN1A rs1059234 and CCND1 rs603965 SNPs were performed in 235 tissue samples from RGA. The association of the genotypes of these two SNPs with the prognosis in the patients with RGA was analyzed by X2 test, multivariate Cox regression analyses, and Kaplan Meier curves.
During the 50 months of median follow-up time, the overall recurrence and survival rate in the whole group was 57.4% and 46.8%, respectively. Whereas, recurrence and survival rate in patients with CC genotype of rs1059234 located in 3'UTR of CDKN1A were 78.0% and 27.1% (p = 0.004; p = 0.006). Multivariate analyses further confirmed that the CC genotype was significantly related with both increased recurrence and death risk (HR 3.33, 95% CI 1.95-5.70; p = 1.07 x 10⁻⁵, and HR 3.45, 95% CI 1.95-6.10; p = 2.03 x 10⁻⁵). No significant difference among CCND1 rs603965 SNP with the prognosis was determined.
rs1059234 of CDKN1A is closely associated with the prognosis in patients with RGA.
细胞周期蛋白依赖性激酶抑制剂1A（CDKN1A）和细胞周期蛋白D1（CCND1）在细胞周期进程调控中发挥重要作用，且与人类癌症密切相关。已证实CDKN1A和CCND1单核苷酸多态性（SNP）会影响不同癌症患者的预后。然而，它们在接受手术切除的胃腺癌（RGA）患者预后中的作用仍有待确定。
对235例RGA组织样本进行CDKN1A rs1059234和CCND1 rs603965 SNP的基因分型。通过X²检验、多因素Cox回归分析和Kaplan Meier曲线分析这两个SNP的基因型与RGA患者预后的相关性。
在中位随访时间50个月期间，全组患者的总复发率和生存率分别为57.4%和46.8%。而位于CDKN1A 3'UTR的rs1059234的CC基因型患者的复发率和生存率分别为78.0%和27.1%（p = 0.004；p = 0.006）。多因素分析进一步证实，CC基因型与复发风险和死亡风险增加均显著相关（HR 3.33，95%CI 1.95 - 5.70；p = 1.07×10⁻⁵，以及HR 3.45，95%CI 1.95 - 6.10；p = 2.03×10⁻⁵）。未确定CCND1 rs603965 SNP与预后之间存在显著差异。
CDKN1A的rs1059234与RGA患者的预后密切相关。
