Hayashi Kazunari, Tabe Yoko, Miida Takashi
Clin Lab. 2016;62(3):471-5. doi: 10.7754/clin.lab.2015.150711.
Circulating microRNA (miRNA) biomarkers have attracted attention as possible blood-based indicators of disease. However, pre-analytic technical issues including serum preparation remain problematic for the clinical translation of such technology. This study investigated the effect of decreased sample preparation time on miRNA measurement.
Blood samples obtained from healthy donors were drawn into blood collection tubes with or without clot activator, and sera were obtained at different preparation time points. The expression levels of miR16 and miR21 were examined.
The miR-16 expression levels were significantly higher in sera separated after 5 minutes compared to ones separated after 10 and 60 minutes, whereas no significant difference of miR21 expression was detected in association with the serum separation conditions.
Serum miRNA expression levels should be interpreted carefully with consideration of the characteristics of collection tubes and clotting status of serum separation.
循环微小RNA（miRNA）生物标志物作为潜在的基于血液的疾病指标已受到关注。然而，包括血清制备在内的分析前技术问题对于此类技术的临床转化仍然存在问题。本研究调查了减少样本制备时间对miRNA测量的影响。
从健康供体采集的血液样本被抽取到含有或不含有凝血激活剂的采血管中，并在不同的制备时间点获取血清。检测miR16和miR21的表达水平。
与10分钟和60分钟后分离的血清相比，5分钟后分离的血清中miR-16表达水平显著更高，而miR21表达与血清分离条件无关，未检测到显著差异。
应结合采血管特性和血清分离的凝血状态谨慎解读血清miRNA表达水平。
