Mohanty Swetaparna, Wu Yang, Chakraborty Nilay, Mohanty Pravansu, Ghosh Gargi
Department of Mechanical Engineering, University of Michigan, Dearborn, 4901 Evergreen Road, Dearborn MI-48128, United States.
Department of Mechanical Engineering, University of Michigan, Dearborn, 4901 Evergreen Road, Dearborn MI-48128, United States.
Mater Sci Eng C Mater Biol Appl. 2016 Aug 1;65:269-77. doi: 10.1016/j.msec.2016.04.055. Epub 2016 Apr 19.
Cryopreservation or cryostorage of tissue engineered constructs can enhance the off-the shelf availability of these products and thus can potentially facilitate the commercialization or clinical translation of tissue engineered products. Encapsulation of cells within hydrogel matrices, in particular alginate, is widely used for fabrication of tissue engineered constructs. While previous studies have explored the cryopreservation response of cells encapsulated within alginate matrices, systematic investigation of the impact of alginate concentration on the metabolic activity and functionality of cryopreserved cells is lacking. The objective of the present work is to determine the metabolic and angiogenic activity of cryopreserved human dermal fibroblasts encapsulated within 1.0%, 1.5% and 2.0% (w/v) alginate matrices. In addition, the goal is to compare the efficacy of dimethyl sulfoxide (DMSO) and trehalose as cryoprotectant. Our study revealed that the concentration of alginate plays a significant role in the cryopreservation response of encapsulated cells. The lowest metabolic activity of the cryopreserved cells was observed in 1% alginate microspheres. When higher concentration of alginate was utilized for cell encapsulation, the metabolic and angiogenic activity of the cells frozen in the absence of cryoprotectants was comparable to that observed in the presence of DMSO or trehalose.
组织工程构建体的冷冻保存或低温储存可以提高这些产品的现货供应能力，从而有可能促进组织工程产品的商业化或临床转化。将细胞封装在水凝胶基质中，特别是海藻酸盐中，被广泛用于制造组织工程构建体。虽然先前的研究已经探讨了封装在海藻酸盐基质中的细胞的冷冻保存反应，但缺乏对海藻酸盐浓度对冷冻保存细胞的代谢活性和功能影响的系统研究。本研究的目的是确定封装在1.0%、1.5%和2.0%（w/v）海藻酸盐基质中的冷冻保存的人真皮成纤维细胞的代谢和血管生成活性。此外，目标是比较二甲基亚砜（DMSO）和海藻糖作为冷冻保护剂的效果。我们的研究表明，海藻酸盐浓度在封装细胞的冷冻保存反应中起着重要作用。在1%海藻酸盐微球中观察到冷冻保存细胞的代谢活性最低。当使用更高浓度的海藻酸盐进行细胞封装时，在没有冷冻保护剂的情况下冷冻的细胞的代谢和血管生成活性与在存在DMSO或海藻糖的情况下观察到的活性相当。
