School of Pharmacy, Guangdong Pharmaceutical University , Guangzhou 510006, China.
J Agric Food Chem. 2016 Jun 1;64(21):4273-9. doi: 10.1021/acs.jafc.6b01580. Epub 2016 May 20.
Durian, known as the king of fruits, is native to Southeast Asia and popular in many countries. Bioactivity-guided fractionation of the peel of durian was applied to determine its bioactive constituents. Four novel phenolics, along with 16 known, were purified and identified. Four novel phenolics were elucidated to be durianol A (1), durianol B (2), durianol C (3), and 5'-methoxy-7'-epi-jatrorin A (4), respectively. The antioxidant and NO inhibitory activities were evaluated for the isolated phenolics. Some phenolics showed significant antioxidant activity in the DPPH and superoxide anion radical scavenging capacity assay. Most of the phenolics revealed pronounced inhibitory effects on NO production in murine RAW 264.7 cells induced by LPS, which showed more potent NO inhibitory activity compared to indomethacin. The results strongly demonstrated that the phenolics may be partially responsible for durian's NO inhibitory activity.
榴莲，被誉为“水果之王”，原产于东南亚，在许多国家广受欢迎。本研究采用生物活性导向分离方法，对榴莲果皮的生物活性成分进行了研究。共分离得到 4 个新的酚类化合物和 16 个已知化合物。通过结构解析，将这 4 个新化合物分别鉴定为榴莲醇 A（1）、榴莲醇 B（2）、榴莲醇 C（3）和 5'-甲氧基-7'-表山荷叶素 A（4）。对分离得到的酚类化合物进行了抗氧化和 NO 抑制活性评价。部分酚类化合物在 DPPH 和超氧阴离子自由基清除能力测定中表现出显著的抗氧化活性。大多数酚类化合物对 LPS 诱导的 RAW 264.7 细胞中 NO 的产生具有明显的抑制作用，其 NO 抑制活性强于吲哚美辛。研究结果表明，酚类化合物可能是榴莲具有 NO 抑制活性的部分原因。
