Muangkram Yuttamol, Amano Akira, Wajjwalku Worawidh, Pinyopummintr Tanu, Thongtip Nikorn, Kaolim Nongnid, Sukmak Manakorn, Kamolnorranath Sumate, Siriaroonrat Boripat, Tipkantha Wanlaya, Maikaew Umaporn, Thomas Warisara, Polsrila Kanda, Dongsaard Kwanreaun, Sanannu Saowaphang, Wattananorrasate Anuwat
a Graduate School of Life Sciences , Ritsumeikan University , Kusatsu , Shiga Japan.
b The Graduate School, Kasetsart University, Jatujak , Bangkok , Thailand.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jul;28(4):597-601. doi: 10.3109/24701394.2016.1149828. Epub 2016 Mar 2.
The Asian tapir (Tapirus indicus) has been classified as Endangered on the IUCN Red List of Threatened Species (2008). Genetic diversity data provide important information for the management of captive breeding and conservation of this species. We analyzed mitochondrial control region (CR) sequences from 37 captive Asian tapirs in Thailand. Multiple alignments of the full-length CR sequences sized 1268 bp comprised three domains as described in other mammal species. Analysis of 16 parsimony-informative variable sites revealed 11 haplotypes. Furthermore, the phylogenetic analysis using median-joining network clearly showed three clades correlated with our earlier cytochrome b gene study in this endangered species. The repetitive motif is located between first and second conserved sequence blocks, similar to the Brazilian tapir. The highest polymorphic site was located in the extended termination associated sequences domain. The results could be applied for future genetic management based in captivity and wild that shows stable populations.
亚洲貘（Tapirus indicus）在《世界自然保护联盟濒危物种红色名录》（2008年）中被列为濒危物种。遗传多样性数据为该物种的圈养繁殖管理和保护提供了重要信息。我们分析了泰国37只圈养亚洲貘的线粒体控制区（CR）序列。全长1268 bp的CR序列的多重比对包含其他哺乳动物物种中描述的三个结构域。对16个简约信息可变位点的分析揭示了11个单倍型。此外，使用中介邻接网络的系统发育分析清楚地显示出三个分支，这与我们早期对该濒危物种的细胞色素b基因研究相关。重复基序位于第一个和第二个保守序列块之间，类似于巴西貘。最高多态性位点位于延伸终止相关序列结构域。这些结果可应用于未来基于圈养和野生环境的遗传管理，以维持稳定的种群数量。
