Luo Xiaotian, Fan Yangbo, Hu Xiaozhong, Miao Miao, Al-Farraj Saleh A, Song Weibo
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, China.
J Eukaryot Microbiol. 2016 Nov;63(6):771-785. doi: 10.1111/jeu.12324. Epub 2016 Jun 1.
The morphology and partial morphogenesis of two freshwater hypotrichous ciliates, Deviata brasiliensis Siqueira-Castro et al., 2009 and Deviata rositae Küppers et al., 2007, isolated from southern China, were investigated using live observation and protargol staining. Our populations resemble the original ones in terms of their live characters and ciliary patterns. The main determinable morphogenetic features of D. brasiliensis basically correspond with those of the type population. However, the origin of anlage V for either proter or opisthe is ambiguous: whether anlage V for the proter originates from parental frontoventral row 2 (the same as in the original population) or parental frontoventral row 3 (the same as in Deviata abbrevescens) or even de novo is not clear; the anlage V for the opisthe is possibly derived from frontoventral row 3 and further migrates to frontoventral row 2, like that in D. abbrevescens. In addition, the SSU rRNA gene was first sequenced for both species. Molecular phylogenetic analyses suggest that the genus Deviata is non-monophyletic and has a close relationship with Perisincirra paucicirrata.
利用活体观察和银染法，对采自中国南方的两种淡水腹毛类纤毛虫——2009年报道的巴西德维纤毛虫（Deviata brasiliensis Siqueira-Castro等）和2007年报道的罗西塔德维纤毛虫（Deviata rositae Küppers等）的形态及部分形态发生过程进行了研究。我们所研究的种群在活体特征和纤毛模式方面与原始种群相似。巴西德维纤毛虫主要的可确定的形态发生特征基本与模式种群一致。然而，前仔虫或后仔虫的第V原基的起源并不明确：前仔虫的第V原基是源自亲代额腹棘毛列2（与原始种群相同）还是亲代额腹棘毛列3（与简略德维纤毛虫相同）甚至是重新发生并不清楚；后仔虫的第V原基可能源自额腹棘毛列3，然后像简略德维纤毛虫那样迁移至额腹棘毛列2。此外，首次对这两个物种的小亚基核糖体RNA（SSU rRNA）基因进行了测序。分子系统发育分析表明，德维纤毛虫属并非单系类群，且与少环周毛虫（Perisincirra paucicirrata）关系密切。
