Dzul-Caamal Ricardo, Hernández-López Abigail, Gonzalez-Jáuregui Mauricio, Padilla Sergio E, Girón-Pérez Manuel Ivan, Vega-López Armando
Laboratorio de Toxicología Ambiental, Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional. Av. Wilfrido Massieu s/n, Unidad Profesional Zacatenco, CP 07738 Distrito Federal, Mexico.
Instituto de Ecología, A.C. (INECOL), Carretera Antigua a Coatepec No. 351, Col. El Haya, C.P. 91070 Xalapa, Veracruz, Mexico.
Comp Biochem Physiol A Mol Integr Physiol. 2016 Oct;200:35-46. doi: 10.1016/j.cbpa.2016.05.006. Epub 2016 May 6.
In this study, we assessed the effects of inorganic and organic pollutants [As, Cu, Fe, Mn, Pb, Zn, PAHs (11 compounds) and total phenols] from a panel of biomarkers [O2, H2O2, thiobarbituric acid reactive substances (TBARS), carbonyl proteins (RCO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total cytochrome P450 activities] evaluated in the Snout Scraping (SS), Serum (S) and Peripheral Blood Cells (PBC) of the Morelet's crocodile (Crocodylus moreletii) inhabiting the reference locality (Lake Mocu) and polluted locality (Champoton River) using Principal Component Analysis (PCA). In male crocodiles from the reference site, only H2O2 in PBC was related to levels of fluoranthene on the Keel of Caudal Scales (KCS), but, in females, no association was detected. In contrast, a sex-linked response was detected in specimens from the polluted locality. Levels of benzo[a]pyrene, benzo[a]anthracene, chrysene, pyrene, phenanthrene, acenaphthene, Zn, Cu, and Pb in KCS of the female crocodil were related to the oxidative stress biomarkers on PBC, incluing the total CYP450 activity and levels of O2, H2O2 in serum. However, in male crocodiles, the oxidative stress in SS and in the serum (TBARS, RCO, CAT, GPx), and SOD in PBC was related to As, Pb, Cu, Fe, and benzo[a]pyrene water concentrations and to the burdens of As, Fe, Mn, indeno[1,2,3cd]pyrene in KCS. These results confirm the usefulness of minimal or non-invasive methods of evaluating the oxidative stress response for the environmental monitoring program on the wild Morelet's crocodile that is subject to special protection in Mexican guidelines.
在本研究中，我们使用主成分分析（PCA）评估了来自一个生物标志物面板[O₂、H₂O₂、硫代巴比妥酸反应性物质（TBARS）、羰基蛋白（RCO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和总细胞色素P450活性]中的无机和有机污染物[砷、铜、铁、锰、铅、锌、多环芳烃（11种化合物）和总酚]，这些生物标志物在生活在参考地点（莫库湖）和污染地点（钱波顿河）的莫雷莱鳄（Crocodylus moreletii）的吻部刮片（SS）、血清（S）和外周血细胞（PBC）中进行了评估。在来自参考地点的雄性鳄鱼中，只有PBC中的H₂O₂与尾鳞龙骨（KCS）上荧蒽的水平有关，但在雌性中未检测到关联。相比之下，在来自污染地点的样本中检测到了性别相关反应。雌性鳄鱼KCS中的苯并[a]芘、苯并[a]蒽、 Chrysene、芘、菲、苊、锌、铜和铅的水平与PBC上的氧化应激生物标志物有关，包括总CYP450活性以及血清中O₂、H₂O₂的水平。然而，在雄性鳄鱼中，SS和血清中的氧化应激（TBARS、RCO、CAT、GPx）以及PBC中的SOD与砷、铅、铜、铁和苯并[a]芘的水浓度以及KCS中砷、铁、锰、茚并[1,2,3 - cd]芘的负荷有关。这些结果证实了在墨西哥指南中受到特别保护的野生莫雷莱鳄的环境监测计划中，使用最小或非侵入性方法评估氧化应激反应的有用性。
