Shea C R, Chen N, Hasan T
Wellman Laboratories of Photomedicine, Department of Dermatology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Lasers Surg Med. 1989;9(2):83-9. doi: 10.1002/lsm.1900090202.
Phototoxicity in cultured human bladder carcinoma cells treated by 514.5-nm argon-ion laser irradiation plus rhodamine-123 (R123) or tetrabromo-R123 (TBR) was assessed counting total cell number and percent viability at 1, 24, 48, 72, and 96 h after irradiation. TBR was markedly more efficient than R123 at causing both reversible and persistent phototoxic cytostasis. Furthermore, TBR photosensitization caused net cytocidal effects at 0.5 J/cm2, which were not seen with R123 photosensitization at up to 40 J/cm2. The reduced phototoxic efficiency of R123 as compared to TBR appears, in part, to reflect the presence of a fraction of cells refractory to R123 photosensitization.
通过514.5纳米氩离子激光照射加罗丹明-123（R123）或四溴-R123（TBR）处理培养的人膀胱癌细胞后的光毒性，通过在照射后1、24、48、72和96小时计数总细胞数和存活百分比来评估。在引起可逆性和持续性光毒性细胞生长停滞方面，TBR比R123明显更有效。此外，TBR光致敏在0.5 J/cm2时引起净杀细胞效应，而高达40 J/cm2的R123光致敏则未观察到这种效应。与TBR相比，R123光毒性效率降低，部分原因似乎是存在一部分对R123光致敏具有抗性的细胞。
