Azam Sadiq, Saqib Muhammad Shahab, Zar Faisal, Ahmad Bashir, Khan Ibrar, Zeb Zermina, Khan Imran
Centre of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
Centre of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan / Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Pak J Pharm Sci. 2016 May;29(3):991-7.
In the current study, the antimicrobial, phytotoxic, haemagglutination and antioxidant potential of crude methanolic extract (Crd. MeOH Ext.) and four organic fractions of Arisaema tortuosum was investigated. All fractions have been screened for antimicrobial properties against eight bacterial pathogens and six fungal pathogens using agar well diffusion and tube dilution method, respectively. Furthermore, the organic fractions were also screened for its phytotoxicity against Lemna minor. Haemagglutination was performed against all human blood groups while free radical scavenging activity was performed to investigate the antioxidant potential of A. tortuosum. Results obtained for antibacterial activity exhibited various degree of zone of inhibition and significant activity was observed for Pseudomonas aeruginosa (27.16±0.60) followed by Bacillus cereus (18.55±0.69) for Crd. MeOH Ext. and chloroform (CHCl3) fraction, respectively while some strains showed resistant at same concentration. Similarly, non-significant antifungal activity was observed for the plant extracts. However, the highest activity among the strains was observed for Alternaria alternata (22±1.24%) and Aspergillus niger (20±1.00%) for ethyl acetate (EtOAc) fraction and Crd. MeOH Ext., respectively. The plant extracts showed good phytotoxic activity with 77.06% inhibition for n-hexane fraction at 1000µg/mL. The result of Nitric Oxide (NO) reducing assay revealed that the plant has less antioxidant activity with 46.06% inhibition for CHCl(3) fraction at 900μg/mL. For haemagglutination assay, the result displayed no agglutination in all the testing concentration. Based on the current results, it can be concluded that A. tortuosum has significant antimicrobial and moderate phytotoxic potential and therefore can leads to antibiotics and herbicide production.
在本研究中，对蛇鞭菊粗甲醇提取物（Crd. MeOH Ext.）及其四个有机组分的抗菌、植物毒性、血凝和抗氧化潜力进行了研究。分别采用琼脂孔扩散法和试管稀释法，对所有组分针对八种细菌病原体和六种真菌病原体的抗菌性能进行了筛选。此外，还对有机组分对小浮萍的植物毒性进行了筛选。对所有人类血型进行了血凝试验，同时进行了自由基清除活性试验以研究蛇鞭菊的抗氧化潜力。抗菌活性结果显示出不同程度的抑菌圈，对于Crd. MeOH Ext.，铜绿假单胞菌（27.16±0.60）表现出显著活性，其次是蜡样芽孢杆菌（18.55±0.69）；对于氯仿（CHCl3）组分，蜡样芽孢杆菌（18.55±0.69）表现出显著活性，而一些菌株在相同浓度下表现出抗性。同样，植物提取物的抗真菌活性不显著。然而，对于乙酸乙酯（EtOAc）组分和Crd. MeOH Ext.，分别对链格孢（22±1.24%）和黑曲霉（20±1.00%）观察到菌株中的最高活性。植物提取物表现出良好的植物毒性活性，在1000µg/mL时正己烷组分的抑制率为77.06%。一氧化氮（NO）还原试验结果表明，该植物的抗氧化活性较低，在900μg/mL时CHCl(3)组分的抑制率为46.06%。对于血凝试验，结果显示在所有测试浓度下均无凝集。基于目前的结果，可以得出结论，蛇鞭菊具有显著的抗菌和中等的植物毒性潜力，因此可用于生产抗生素和除草剂。
