Fang Shisong, Wang Xin, Dong Fangyuan, Jin Tao, Liu Guang, Lu Xing, Peng Bo, Wu Weihua, Liu Hui, Kong Dongfeng, Tang Xiujuan, Qin Yanmin, Mei Shujiang, Xie Xu, He Jianfan, Ma Hanwu, Zhang Renli, Cheng Jinquan
Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, No. 8, Longyuan Road, Nanshan District, Shenzhen, Guangdong, China.
College of Medicine, Jinan University, 601 Huangpu Avenue West, Guangzhou, China.
Arch Virol. 2016 Aug;161(8):2117-32. doi: 10.1007/s00705-016-2872-1. Epub 2016 May 11.
There were three epidemic waves of human infection with avian influenza A (H7N9) virus in 2013-2014. While many analyses of the genomic origin, evolution, and molecular characteristics of the influenza A (H7N9) virus have been performed using sequences from the first epidemic wave, genomic characterization of the virus from the second epidemic wave has been comparatively less reported. In this study, an in-depth analysis was performed with respect to the genomic characteristics of 11 H7N9 virus strains isolated from confirmed cases and four H7N9 virus strains isolated from environmental samples in Shenzhen during the second epidemic wave. Phylogenetic analysis demonstrated that six internal segments of the influenza A (H7N9) virus isolated from confirmed cases and environmental samples in Shenzhen were clustered into two different clades and that the origin of the influenza A (H7N9) virus isolated from confirmed cases in Shenzhen was different from that of viruses isolated during the first wave. In addition, H9N2 viruses, which were prevalent in southern China, played an important role in the reassortment of the influenza A (H7N9) virus isolated in Shenzhen. HA-R47K and -T122A, PB2-V139I, PB1-I397M, and NS1-T216P were the signature amino acids of the influenza A (H7N9) virus isolated from confirmed cases in Shenzhen. We found that the HA, NA, M, and PA genes of the A(H7N9) viruses underwent positive selection in the human population. Therefore, enhanced surveillance should be carried out to determine the origin and mode of transmission of the novel influenza A (H7N9) virus and to facilitate the formulation of effective policies for prevention and containment of a human infection epidemics.
2013年至2014年期间出现了三波人感染甲型H7N9禽流感病毒疫情。虽然已利用第一波疫情的序列对甲型H7N9禽流感病毒的基因组起源、进化和分子特征进行了许多分析，但关于第二波疫情病毒的基因组特征报道相对较少。在本研究中，对深圳第二波疫情期间从确诊病例中分离出的11株H7N9病毒株和从环境样本中分离出的4株H7N9病毒株的基因组特征进行了深入分析。系统发育分析表明，从深圳确诊病例和环境样本中分离出的甲型H7N9禽流感病毒的6个内部片段聚为两个不同的进化枝，且从深圳确诊病例中分离出的甲型H7N9禽流感病毒的起源与第一波疫情期间分离出的病毒不同。此外，在中国南方流行的H9N2病毒在深圳分离出的甲型H7N9禽流感病毒的重配中发挥了重要作用。HA-R47K和-T122A、PB2-V139I、PB1-I397M以及NS1-T216P是从深圳确诊病例中分离出的甲型H7N9禽流感病毒的特征性氨基酸。我们发现A(H7N9)病毒的HA、NA、M和PA基因在人群中经历了正选择。因此，应加强监测以确定新型甲型H7N9禽流感病毒的起源和传播方式，并促进制定有效的预防和控制人感染疫情的政策。
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