不同激活剂对体外成熟的山羊卵母细胞激活发育的影响。
Effect of different activators on development of activated in vitro matured caprine oocytes.
作者信息
Sharma J R, Agarwal S, Kharche S D, Goel A K, Jindal S K, Agarwal S K
机构信息
MSc, Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India;
Ph.D. Scholar, Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India;
出版信息
Iran J Vet Res. 2015 Winter;16(1):42-6.
This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC's) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oocytes (n=226) were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa medium. Group 2 in vitro matured oocytes (n=294) were exposed to 7% ethanol for 5 min followed by treatment with 10 µg/ml CHX for 4 h in mCR2aa medium. Group 3 in vitro matured oocytes (n=325) were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP and 10 µg/ml CHX for 4 h in mCR2aa medium. Group 4 in vitro matured oocytes (n=108) were cultured for 4 h without any chemical treatment in mCR2aa medium (control). The cleavage rate in groups 1, 2, 3 and 4 was 54.42%, 44.55%, 51.69% and 0.00%, respectively. The percentage of morula and blastocyst production in group 1, group 2 and group 3 was 26.01%, 29.77% and 29.76% and 2.43%, 1.52% and 1.78%, respectively. These results suggest that the activation of in vitro matured oocytes by 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa is most favorable for parthenogenetic caprine embryos production.
本研究旨在比较不同激活处理对体外成熟卵母细胞的激活效果及其在mCR2aa培养基中的发育潜能，以获得最大数量的胚胎。从480个卵巢中总共收集了1090个卵丘卵母细胞复合体（COC）。体外成熟的卵母细胞被随机分为四组。第1组体外成熟卵母细胞（n = 226）在mCR2aa培养基中用7%乙醇处理5分钟，然后用2.0 mM二甲氨基嘌呤（DMAP）处理4小时。第2组体外成熟卵母细胞（n = 294）在mCR2aa培养基中用7%乙醇处理5分钟，然后用10 µg/ml环己酰亚胺（CHX）处理4小时。第3组体外成熟卵母细胞（n = 325）在mCR2aa培养基中用7%乙醇处理5分钟，然后用2.0 mM DMAP和10 µg/ml CHX处理4小时。第4组体外成熟卵母细胞（n = 108）在mCR2aa培养基中不进行任何化学处理培养4小时（对照组）。第1、2、3和4组的卵裂率分别为54.42%、44.55%、51.69%和0.00%。第1、2和3组桑葚胚和囊胚产生的百分比分别为26.01%、29.77%和29.76%，以及2.43%、1.52%和1.78%。这些结果表明，在mCR2aa培养基中用7%乙醇处理体外成熟卵母细胞5分钟，然后用2.0 mM DMAP处理4小时，最有利于孤雌山羊胚胎的产生。
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