Zarik Lamia, Meddich Abdelilah, Hijri Mohamed, Hafidi Mohamed, Ouhammou Ahmed, Ouahmane Lahcen, Duponnois Robin, Boumezzough Ali
Laboratoire Écologie et Environnement (unité associée au CNRST URAC32, unité associée au CNERS), Faculté des sciences Semlalia, Université Cadi-Ayyad, Marrakech, Morocco.
Institut de recherche en biologie végétale (IRVB), Université de Montréal, Montréal, QC, Canada.
C R Biol. 2016 May-Jun;339(5-6):185-196. doi: 10.1016/j.crvi.2016.04.009. Epub 2016 May 11.
In this study, we investigated whether indigenous arbuscular mycorrhizal (AM) fungi could improve the tolerance of Cupressus atlantica against water deficit. We tested a gradient of watering regime spanning from 90% to 25% of soil retention capacity of water on mycorhized and non-mycorhized seedlings in pot cultures with sterilized and non-sterilized soils. Our result showed a positive impact of AM fungi on shoot height, stem diameter and biomass as well as on the growth rate. We also observed that inoculation with AM fungi significantly improved uptake of minerals by C. atlantica in both sterilized and non-sterilized soils independently of water regimes. We found that mycorhized plants maintained higher relative water content (RWC) and water potential compared with non-mycorhized plants that were subjected to drought-stress regimes (50% and 25% of soil retention capacity). The contents of proline and of soluble sugars showed that their concentrations decreased in non-mycorhized plants subjected to DS. Superoxide dismutase (SOD) and catalase (CAT) activities also decreased in non-mycorhized plants submitted to DS compared to mycorhized plants. The same pattern was observed by measuring peroxidase (POD) enzyme activity. The results demonstrated that AM fungal inoculation promoted the growth and tolerance of C. atlantica against DS in pot cultures. Therefore, mycorrhizal inoculation could be a potential solution for the conservation and reestablishment of C. atlantica in its natural ecosystem.
在本研究中，我们调查了本土丛枝菌根（AM）真菌是否能提高大西洋柏对水分亏缺的耐受性。我们在盆栽试验中，对接种和未接种AM真菌的幼苗，采用了从土壤持水量的90%到25%的不同浇水梯度，并使用了灭菌和未灭菌的土壤。我们的结果表明，AM真菌对株高、茎直径、生物量以及生长速率都有积极影响。我们还观察到，无论水分状况如何，在灭菌和未灭菌的土壤中，接种AM真菌都能显著提高大西洋柏对矿物质的吸收。我们发现，与遭受干旱胁迫（土壤持水量的50%和25%）的未接种AM真菌的植物相比，接种AM真菌的植物保持了更高的相对含水量（RWC）和水势。脯氨酸和可溶性糖的含量表明，在遭受干旱胁迫的未接种AM真菌的植物中，它们的浓度降低。与接种AM真菌的植物相比，遭受干旱胁迫的未接种AM真菌的植物中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性也降低。通过测量过氧化物酶（POD）活性也观察到了相同的模式。结果表明，在盆栽试验中，接种AM真菌促进了大西洋柏的生长并提高了其对干旱胁迫的耐受性。因此，菌根接种可能是在其自然生态系统中保护和重建大西洋柏的一种潜在解决方案。
