Wang Shan, Li Heng, Zhang Min, Yue Long-Tao, Wang Cong-Cong, Zhang Peng, Liu Ying, Duan Rui-Sheng
Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR China.
Central Laboratory, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, PR China.
Neurosci Lett. 2016 Jul 28;626:25-34. doi: 10.1016/j.neulet.2016.05.020. Epub 2016 May 12.
Curcumin is a traditional Asian medicine with diverse immunomodulatory properties used therapeutically in the treatment of many autoimmune diseases. However, the effects of curcumin on myasthenia gravis (MG) remain undefined. Here we investigated the effects and potential mechanisms of curcumin in experimental autoimmune myasthenia gravis (EAMG). Our results demonstrated that curcumin ameliorated the clinical scores of EAMG, suppressed the expression of T cell co-stimulatory molecules (CD80 and CD86) and MHC class II, down-regulated the levels of pro-inflammatory cytokines (IL-17, IFN-γ and TNF-α) and up-regulated the levels of the anti-inflammatory cytokine IL-10, shifted the balance from Th1/Th17 toward Th2/Treg, and increased the numbers of NKR-P1(+) cells (natural killer cell receptor protein 1 positive cells, including NK and NKT cells). Moreover, the administration of curcumin promoted the differentiation of B cells into a subset of B10 cells, increased the anti-R97-166 peptide IgG1 levels and decreased the relative affinity indexes of anti-R97-116 peptide IgG. In summary, curcumin effectively ameliorate EAMG, indicating that curcumin may be a potential candidate therapeutic agent for MG.
姜黄素是一种具有多种免疫调节特性的传统亚洲药物，在治疗多种自身免疫性疾病中具有治疗作用。然而，姜黄素对重症肌无力（MG）的影响仍不明确。在此，我们研究了姜黄素在实验性自身免疫性重症肌无力（EAMG）中的作用及潜在机制。我们的结果表明，姜黄素改善了EAMG的临床评分，抑制了T细胞共刺激分子（CD80和CD86）和MHC II类分子的表达，下调了促炎细胞因子（IL-17、IFN-γ和TNF-α）的水平，上调了抗炎细胞因子IL-10的水平，使平衡从Th1/Th17向Th2/Treg转变，并增加了NKR-P1(+)细胞（自然杀伤细胞受体蛋白1阳性细胞，包括NK和NKT细胞）的数量。此外，给予姜黄素促进B细胞分化为B10细胞亚群，增加抗R97-166肽IgG1水平，并降低抗R97-116肽IgG的相对亲和指数。总之，姜黄素有效地改善了EAMG，表明姜黄素可能是MG的一种潜在候选治疗药物。
