辣木籽凝集素作为水溶液中金属去除剂的评估。
Evaluation of Moringa oleifera Seed Lectin as a Metal Remover in Aqueous Solutions.
作者信息
Freitas José H E S, de Santana Keissy V, da Silva Pollyanna M, de Moura Maiara C, Coelho Luana C B B, do Nascimento Aline E, Paiva Patrícia M G, Napoleao Thiago H
机构信息
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Pernambuco, Brazil.
出版信息
Protein Pept Lett. 2016;23(7):645-9. doi: 10.2174/0929866523666160517123558.
The efficacy of Moringa oleifera seed lectin (WSMoL) as a metal remover in water and the effects of metals on its hemagglutinating and antibacterial activities were determined. Aqueous metal solutions were incubated with WSMoL for 8 h at 4°C and the concentrations of metals were determined using atomic absorption spectroscopy. Hemagglutination and antibacterial assays were conducted with WSMoL and lectin exposed or not to the metals. The removal efficiency of WSMoL was 49.00%, 53.21%, 71.45%, 55.42%, 69.88%, 62.14%, and 49.36% for Cd+2, Pb+2, Cu+2, Zn+2, Mg+2, Mn+2, and Al+3, respectively. WSMoL showed bacteriostatic and bactericidal activities against Escherichia coli and Salmonella enterica serovar Enteritidis. However, hemagglutinating and antibacterial activities were impaired after exposure to metals. In conclusion, WSMoL efficiently removed metals present in water but the interaction with metals impaired lectin carbohydrate-binding ability and antibacterial activity. This should be considered when properties of WSMoL other than metal removal are desired.
测定了辣木籽凝集素（WSMoL）作为水中金属去除剂的功效以及金属对其血凝和抗菌活性的影响。将金属水溶液与WSMoL在4°C下孵育8小时，并用原子吸收光谱法测定金属浓度。对暴露于金属或未暴露于金属的WSMoL进行血凝和抗菌试验。WSMoL对Cd+2、Pb+2、Cu+2、Zn+2、Mg+2、Mn+2和Al+3的去除效率分别为49.00%、53.21%、71.45%、55.42%、69.88%、62.14%和49.36%。WSMoL对大肠杆菌和肠炎沙门氏菌肠炎血清型具有抑菌和杀菌活性。然而，暴露于金属后，血凝和抗菌活性受到损害。总之，WSMoL能有效去除水中存在的金属，但与金属的相互作用会损害凝集素的碳水化合物结合能力和抗菌活性。当需要WSMoL除金属去除之外的其他特性时，应考虑这一点。
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