Coriolano Marilia C, de Santana Brito Jessica, de Siqueira Patriota Leydianne L, de Araujo Soares Ana K, de Lorena Virginia M B, Paiva Patricia M G, Napoleao Thiago H, Coelho Luana C B B, de Melo Cristiane M L
Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Centro de Pesquisas Aggeu Magalhaes, Fundacao Oswaldo Cruz, Recife, Pernambuco, Brazil.
Protein Pept Lett. 2018;25(3):295-301. doi: 10.2174/0929866525666180130141736.
Moringa oleifera is used in traditional medicine as well as in food, cosmetic, and pharmaceutical industries. Water-soluble M. oleifera lectin (WSMoL) is an anionic protein isolated from the seeds of this tree. Until now, immune responses promoted by this lectin in human PBMC have not been investigated.
The main objective of this study was to investigate the immunomodulatory effects of WSMoL on human PBMC through measurement of lymphocytes subsets, cytokine and nitric oxide levels.
Human peripheral blood mononuclear cells (PBMC) were isolated through Ficoll technique, were incubated with WSMoL (10 µg/mL) for 24, 48 and 72 hours, and was performed immunophenotyping assay of lymphocytes and monocytes. Culture supernatants were used to determined cytokine and nitric oxide levels. Assays with cells subsets and cytokine production were performed through cytometry. Nitric oxide release assay was determinate by spectrophotometry.
WSMoL induced the release of the cytokines TNF-α, IL-2, IL-6, IL-10 as well as nitric oxide. Incubation of PBMC with this lectin also led to activation of CD8+ T lymphocytes.
WSMoL promotes immunomodulation in human PBMC inducing a potential wound healing profile and, in future in vivo assays, can be evaluated as adjuvant in immunosuppressive diseases and wound repair.
辣木在传统医学以及食品、化妆品和制药行业中都有应用。水溶性辣木凝集素（WSMoL）是从这种树的种子中分离出的一种阴离子蛋白。到目前为止，这种凝集素在人外周血单个核细胞（PBMC）中引发的免疫反应尚未得到研究。
本研究的主要目的是通过测量淋巴细胞亚群、细胞因子和一氧化氮水平来研究WSMoL对人PBMC的免疫调节作用。
通过Ficoll技术分离人外周血单个核细胞（PBMC），将其与WSMoL（10 µg/mL）孵育24、48和72小时，并对淋巴细胞和单核细胞进行免疫表型分析。培养上清液用于测定细胞因子和一氧化氮水平。通过细胞计数法对细胞亚群和细胞因子产生进行检测。一氧化氮释放检测通过分光光度法进行测定。
WSMoL诱导细胞因子TNF-α、IL-2、IL-6、IL-10以及一氧化氮的释放。PBMC与这种凝集素孵育还导致CD8 + T淋巴细胞的激活。
WSMoL在人PBMC中促进免疫调节，诱导潜在的伤口愈合特征，在未来的体内试验中，可作为免疫抑制疾病和伤口修复的佐剂进行评估。
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