Han Min-Eui, Kim Su-Hwan, Kim Hwan D, Yim Hyun-Gu, Bencherif Sidi A, Kim Tae-Il, Hwang Nathaniel S
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of Korea.
School of Chemical and Biological Engineering, Institute of Chemical Processes, N-Bio Institute, Seoul National University, Seoul, Republic of Korea.
Int J Biol Macromol. 2016 Dec;93(Pt B):1410-1419. doi: 10.1016/j.ijbiomac.2016.05.024. Epub 2016 May 13.
In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical polymerization under freezing conditions. This procedure induces ice crystallization (used as a porogen) prior polymer crosslinking in which, after complete cryopolymerization, a thawing process transforms the ice crystals into a unique interconnected macroporous structure within ECM-cryogels. The developed ECM-cryogels exhibited an average macroporosity of 75% and supported the infiltration of chondrocyteds. When rabbit chondrocytes were cultured on ECM-cryogels, MeCS-based cryogels stimulated aggrecan gene expression and GAG accumulation, whereas MeHA-based cryogels stimulated type II collagen gene expression and collagen accumulation. These results demonstrate that design of ECM-based cryogels can play an important role in promoting specific ECM proteins secretion for cartilage tissue engineering.
在本研究中，我们研究了各种用于软骨组织工程的基于细胞外基质（ECM）的高度多孔冷冻凝胶。为了制备基于ECM的冷冻凝胶，甲基丙烯酸化硫酸软骨素（MeCS）或甲基丙烯酸化透明质酸（MeHA）与聚（乙二醇）二丙烯酸酯（PEGDA）在冷冻条件下通过自由基聚合交联。该过程在聚合物交联之前诱导冰结晶（用作致孔剂），其中在完全冷冻聚合后，解冻过程将冰晶转化为ECM冷冻凝胶内独特的相互连接的大孔结构。所开发的ECM冷冻凝胶表现出75%的平均大孔率，并支持软骨细胞的浸润。当兔软骨细胞在ECM冷冻凝胶上培养时，基于MeCS的冷冻凝胶刺激聚集蛋白聚糖基因表达和糖胺聚糖积累，而基于MeHA的冷冻凝胶刺激II型胶原基因表达和胶原积累。这些结果表明，基于ECM的冷冻凝胶的设计在促进软骨组织工程中特定ECM蛋白的分泌方面可以发挥重要作用。
