Hamano Y, Kurimoto Y
a Laboratory of Animal Nutrition, Field Education and Science Center, Faculty of Bioresource Sciences , Akita Prefectural University , Ohgata , Akita , Japan.
b Institute of Wood Technology , Akita Prefectural University , Noshiro , Akita , Japan.
Br Poult Sci. 2016 Oct;57(5):643-654. doi: 10.1080/00071668.2016.1190809. Epub 2016 Aug 11.
The present study was conducted to determine the effects of acetylated wood powder (AW) as a new feed additive on performance, liver and muscle metabolism of amino acids and fatty acids and nucleotide-related substances of meat in broiler chickens. It was hypothesised that acetic acid desorbed from AW during intestinal digestion affects tissue metabolism. Two-week-old broiler chicks were divided into four groups and fed on diets supplemented with wood powder (30 g/kg) less than 106 µm in diameter, except for controls. The AW was added to diets at 0, 10 and 30 g/kg to replace the non-acetylated wood powder (NAW) for 26 d. Plasma, liver tissue and breast muscle were taken from half of birds at 40 d of age under the fed condition. After the remaining chickens were fasted for 14 h, breast muscle was taken and refrigerated for 24 h. Consumption of wood powder with or without acetyl groups had no effect on growth performance including tissue weights of abdominal fat and breast muscle and plasma metabolites. Feeding AW decreased total free amino acid concentrations in the liver compared to the group only fed on the NAW. This response was dependent mainly on reduced non-essential and glucogenic amino acid concentrations. However, in breast muscle, alterations of free amino acid concentrations were observed only for histidine and tryptophan. In addition, the fatty acid composition of liver and breast muscle was not affected by feeding AW. In breast meat obtained from fasted chickens, the higher level of AW increased the concentration of inosine 5'-monophosphate, a taste-active compound, and in contrast, decreased the subsequent catabolites (inosine and hypoxanthine). However, the concentration of glutamic acid, a taste-active compound, was lowered at this level of AW ingestion. Therefore, this study suggested that feeding AW as a new feed additive regulates ante-mortem amino acid utilisation in the liver and contributes to retard post-mortem degradation of inosine 5'-monophosphate as a taste-active compound in chicken meat.
本研究旨在确定新型饲料添加剂乙酰化木粉（AW）对肉鸡生产性能、肝脏和肌肉中氨基酸及脂肪酸代谢以及肉中核苷酸相关物质的影响。研究假设肠道消化过程中从AW解吸的乙酸会影响组织代谢。将两周龄的肉鸡分为四组，除对照组外，其余各组饲喂添加了直径小于106 µm木粉（30 g/kg）的日粮。将AW以0、10和30 g/kg的剂量添加到日粮中，替代未乙酰化木粉（NAW），持续26天。在40日龄时，在饲喂状态下从半数鸡采集血浆、肝脏组织和胸肌。其余鸡禁食14小时后，采集胸肌并冷藏24小时。摄入含或不含乙酰基的木粉对生长性能（包括腹脂和胸肌组织重量以及血浆代谢物）没有影响。与仅饲喂NAW的组相比，饲喂AW可降低肝脏中总游离氨基酸浓度。这种反应主要取决于非必需氨基酸和生糖氨基酸浓度的降低。然而，在胸肌中，仅组氨酸和色氨酸的游离氨基酸浓度发生了变化。此外，肝脏和胸肌的脂肪酸组成不受饲喂AW的影响。在禁食鸡的胸肉中，较高水平的AW增加了呈味活性化合物5'-肌苷酸的浓度，相反，降低了其后续分解代谢产物（肌苷和次黄嘌呤）的浓度。然而，在这种AW摄入水平下，呈味活性化合物谷氨酸的浓度降低。因此，本研究表明，将AW作为新型饲料添加剂饲喂可调节肝脏宰前氨基酸利用，并有助于延缓鸡肉中呈味活性化合物5'-肌苷酸的宰后降解。
