Norstedt G, Levinovitz A, Eriksson H
Department of Medical Nutrition, Huddinge University Hospital, Karolinska Institute, Sweden.
Acta Endocrinol (Copenh). 1989 Apr;120(4):466-72. doi: 10.1530/acta.0.1200466.
IGF-I and IGF-II are peptides with mitogenic properties. In this study mRNA for IGF-I and IGF-II was analysed in rat uterine tissue after different endocrine manipulations and the possibility of an estrogenic regulation of IGF expression was investigated. Both IGF-I and IGF-II mRNA were present in uterine tissue. The level of IGF-I mRNA, but not IGF-II mRNA, was reduced following ovariectomy. Administration of estradiol (2.5 micrograms/day for 4 days) to ovariectomized rats increased IGF-I mRNA 8-fold to levels seen in intact animals. In adult animals hepatic IGF-I mRNA did not appear to be increased by estrogen treatment. Low levels of IGF-II mRNA were detected in the uterus, but showed no dependence on estrogen. The inductive effect of estrogen on uterine IGF-I mRNA could not be substituted for by growth hormone administration (0.5 mg/100 g, ip for 6 h). The present results suggest IGF-I as a potential candidate for a mediator of estrogen-induced growth. Both estrogen and GH induce IGF-I mRNA and a tissue specificity for these hormones is indicated where GH regulates hepatic and estrogen uterine IGF-I mRNA.
胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子-II（IGF-II）是具有促有丝分裂特性的肽。在本研究中，分析了不同内分泌处理后大鼠子宫组织中IGF-I和IGF-II的信使核糖核酸（mRNA），并研究了雌激素对IGF表达进行调节的可能性。子宫组织中同时存在IGF-I和IGF-II的mRNA。卵巢切除术后，IGF-I的mRNA水平降低，但IGF-II的mRNA水平未降低。给去卵巢大鼠注射雌二醇（2.5微克/天，共4天）可使IGF-I的mRNA增加8倍，达到完整动物中的水平。在成年动物中，雌激素处理似乎并未增加肝脏中IGF-I的mRNA。子宫中检测到低水平的IGF-II的mRNA，但未显示出对雌激素的依赖性。生长激素给药（0.5毫克/100克，腹腔注射6小时）不能替代雌激素对子宫IGF-I的mRNA的诱导作用。目前的结果表明，IGF-I可能是雌激素诱导生长的一种潜在介质。雌激素和生长激素均诱导IGF-I的mRNA，并且这些激素存在组织特异性，即生长激素调节肝脏中的IGF-I的mRNA，而雌激素调节子宫中的IGF-I的mRNA。
