西尼罗河病毒在小鼠模型中枢神经系统中的神经侵袭和神经发病机制研究

Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model.

作者信息

Sultana Hameeda

机构信息

Center for Molecular Medicine, Department of Biological Sciences, Old Dominion University, Norfolk, VA, 23529-0266, USA.

出版信息

Methods Mol Biol. 2016;1435:83-101. doi: 10.1007/978-1-4939-3670-0_8.

DOI:10.1007/978-1-4939-3670-0_8

PMID:27188552

Abstract

West Nile virus (WNV) is a neurotropic virus that causes inflammation and neuronal loss in the Central Nervous System leading to encephalitis and death. In this chapter, detailed methods to detect WNV in the murine brain tissue by quantitative real-time polymerase chain reaction and viral plaque assays are described. Determination of WNV neuropathogenesis by Hematoxylin and Eosin staining and immunohistochemical procedures are provided. In addition, TUNEL assays to determine neuronal loss during WNV neuropathogenesis are discussed in detail. Collectively, the methods mentioned in this chapter provide an overview to understand neuroinvasion and neuropathogenesis in a murine model of WNV infection.

摘要

西尼罗河病毒（WNV）是一种嗜神经病毒，可导致中枢神经系统炎症和神经元丧失，进而引发脑炎和死亡。在本章中，将描述通过定量实时聚合酶链反应和病毒蚀斑试验在小鼠脑组织中检测WNV的详细方法。还提供了通过苏木精和伊红染色以及免疫组织化学程序来确定WNV神经发病机制的方法。此外，还将详细讨论用于确定WNV神经发病机制过程中神经元丧失的TUNEL试验。总体而言，本章所述方法为了解WNV感染小鼠模型中的神经侵袭和神经发病机制提供了一个概述。