鉴定西尼罗河病毒感染所涉及宿主因素的方法学

Methodology for Identifying Host Factors Involved in West Nile Virus Infection.

作者信息

Krishnan Manoj N

机构信息

Signature Research Program - Emerging Infectious Diseases, Duke-NUS Graduate Medical School, National University of Singapore, 8 College Road, Singapore, 169857, Singapore.

出版信息

Methods Mol Biol. 2016;1435:115-27. doi: 10.1007/978-1-4939-3670-0_10.

DOI:10.1007/978-1-4939-3670-0_10

PMID:27188554

Abstract

The West Nile virus (WNV) infection is a major medical problem for humans and some domesticated animals. WNV infection of host cells involves the interplay of the virus with several host factors. Identification of the host factors impacting on WNV infection can enhance our understanding of virus infection mechanisms, host immune defense mechanisms, and also reveal novel host targets that can be developed as antivirals. RNA interference (RNAi) is a highly efficient genetic tool to discover host genes involved in WNV infection at a genome scale. Here, we describe a protocol for conducting human genome wide RNAi screen to discover novel host factors associated with WNV infection of human cells.

摘要

西尼罗河病毒（WNV）感染是人类和一些家畜面临的一个主要医学问题。宿主细胞的WNV感染涉及病毒与多种宿主因子的相互作用。鉴定影响WNV感染的宿主因子可以增进我们对病毒感染机制、宿主免疫防御机制的理解，还能揭示可作为抗病毒药物开发的新宿主靶点。RNA干扰（RNAi）是一种高效的遗传工具，可在基因组规模上发现参与WNV感染的宿主基因。在此，我们描述了一种进行人类全基因组RNAi筛选的方案，以发现与人类细胞WNV感染相关的新宿主因子。