Horiguchi Ikki, Sakai Yasuyuki
Dept. of Chemical System Engineering, The University of Tokyo, Tokyo, Japan.
Center for International Research on Integrative Biomedical Systems, Inst. of Industrial Science, The University of Tokyo, Tokyo, Japan.
Biotechnol Prog. 2016 Jul 8;32(4):1009-16. doi: 10.1002/btpr.2301. Epub 2016 Jun 14.
Suspension culture systems are currently under investigation for the mass production of pluripotent stem (PS) cells for tissue engineering; however, the control of cell aggregation in suspension culture remains challenging. Existing methods to control aggregation such as microwell culture are difficult to scale up. To address this issue, in this study a novel method that incorporates the addition of KnockOut Serum Replacement (KSR) to the PS cell culture medium was described. The method regulated cellular aggregation and significantly improved cell growth (a 2- to 10-fold increase) without any influence on pluripotency. In addition, albumin-associated lipids as the major working ingredient of KSR responsible for this inhibition of aggregation were identified. This is one of the simplest methods described to date to control aggregation and requires only chemically synthesizable reagents. Thus, this method has the potential to simplify the mass production process of PS cells and thus lower their cost. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1009-1016, 2016.
目前，悬浮培养系统正在用于大规模生产用于组织工程的多能干细胞（PS细胞）；然而，悬浮培养中细胞聚集的控制仍然具有挑战性。现有的控制聚集的方法，如微孔培养，难以扩大规模。为了解决这个问题，在本研究中描述了一种新方法，即在PS细胞培养基中添加敲除血清替代物（KSR）。该方法调节细胞聚集并显著改善细胞生长（增加2至10倍），而对多能性没有任何影响。此外，确定了作为KSR主要有效成分的白蛋白相关脂质是造成这种聚集抑制的原因。这是迄今为止所描述的控制聚集的最简单方法之一，只需要化学合成试剂。因此，该方法有可能简化PS细胞的大规模生产过程，从而降低其成本。©2016美国化学工程师学会生物技术进展，32:1009 - 1016,2016。
