嗜温明串珠菌K7产生的白细胞素K7对单核细胞增生李斯特菌的作用模式及其在牛奶保鲜中的潜力。
Mode of action of leucocin K7 produced by Leuconostoc mesenteroides K7 against Listeria monocytogenes and its potential in milk preservation.
作者信息
Shi Feng, Wang Yanwei, Li Yongfu, Wang Xiaoyuan
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu, Wuxi, 214122, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
出版信息
Biotechnol Lett. 2016 Sep;38(9):1551-7. doi: 10.1007/s10529-016-2127-y. Epub 2016 May 18.
OBJECTIVES
To investigate the mode of action of leucocin K7 against Listeria monocytogenes and to assess its inhibitory effect on Lis. monocytogenes in refrigerated milk.
RESULTS
A bacteriocin-producing strain, Leuconostoc mesenteroides K7, was isolated from a fermented pickle. The bacteriocin, leucocin K7, exhibited antagonistic activity against Lis. monocytogenes with an MIC of 28 µg/ml. It was sensitive to proteaseS and displayed good thermal stability and broad active pH range. Leucocin K7 had no effect on the efflux of ATP from Lis. monocytogenes but triggered the efflux of K(+) and the intracellular hydrolysis of ATP. It also dissipated the transmembrane electrical potential completely and transmembrane pH gradient partially. It 80 AU/ml inhibited the growth of Lis. monocytogenes by 2.3-3.9 log units in milk; when combined with glycine (5 mg/ml), it completely eliminated viable Lis. monocytogenes over 7 days
CONCLUSION
Leucocin K7 shows different mode of action from nisin and may have potential application in milk preservation.
目的
研究白细胞素K7对单核细胞增生李斯特菌的作用模式，并评估其对冷藏牛奶中单核细胞增生李斯特菌的抑制效果。
结果
从发酵泡菜中分离出一株产细菌素的菌株——肠膜明串珠菌K7。该细菌素白细胞素K7对单核细胞增生李斯特菌具有拮抗活性，最小抑菌浓度为28微克/毫升。它对蛋白酶敏感，具有良好的热稳定性和较宽的活性pH范围。白细胞素K7对单核细胞增生李斯特菌的ATP外排没有影响，但会引发钾离子外流和ATP的细胞内水解。它还能完全消除跨膜电势，并部分消除跨膜pH梯度。80 AU/ml的白细胞素K7可使牛奶中单核细胞增生李斯特菌的生长抑制2.3 - 3.9个对数单位；与甘氨酸（5毫克/毫升）联合使用时，可在7天内完全消除单核细胞增生李斯特菌活菌。
结论
白细胞素K7显示出与乳链菌肽不同的作用模式，可能在牛奶保鲜中有潜在应用。
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