Papathanasopoulos M A, Krier F, Revol-Junelles A M, Lefebvre G, Le Caer J P, von Holy A, Hastings J W
Department of Microbiology, University of the Witwatersrand, South Africa.
Curr Microbiol. 1997 Dec;35(6):331-5. doi: 10.1007/s002849900264.
Leuconostoc (Lc.) mesenteroides TA33a produced three bacteriocins with different inhibitory activity spectra. Bacteriocins were purified by adsorption/desorption from producer cells and reverse phase high-performance liquid chromatography. Leucocin C-TA33a, a novel bacteriocin with a predicted molecular mass of 4598 Da, inhibited Listeria and other lactic acid bacteria (LAB). Leucocin B-TA33a has a predicted molecular mass of 3466 Da, with activity against Leuconostoc/Weissella (W.) strains, and appears similar to mesenterocin 52B and dextranicin 24, while leucocin A-TA33a, which also inhibited Listeria and other LAB strains, is identical to leucocin A-UAL 187. A survey of other known bacteriocin-producing Leuconostoc/Weissella strains for the presence of the three different bacteriocins revealed that production of leucocin A-, B- and C-type bacteriocins was widespread. Lc. carnosum LA54a, W. paramesenteroides LA7a, and Lc. gelidum UAL 187-22 produced all three bacteriocins, whereas W. paramesenteroides OX and Lc. carnosum TA11a produced only leucocin A- and B-type bacteriocins.
嗜柠檬酸明串珠菌（Leuconostoc，Lc.）TA33a产生了三种具有不同抑制活性谱的细菌素。细菌素通过从产生菌细胞中吸附/解吸以及反相高效液相色谱法进行纯化。Leucocin C-TA33a是一种预测分子量为4598 Da的新型细菌素，可抑制李斯特菌和其他乳酸菌（LAB）。Leucocin B-TA33a预测分子量为3466 Da，对明串珠菌/魏斯氏菌（Weissella，W.）菌株具有活性，且似乎与肠膜菌素52B和葡聚糖素24相似，而同样抑制李斯特菌和其他LAB菌株的Leucocin A-TA33a与Leucocin A-UAL 187相同。对其他已知产生细菌素的明串珠菌/魏斯氏菌菌株进行这三种不同细菌素的检测发现，Leucocin A、B和C型细菌素的产生很普遍。肉明串珠菌（Lc. carnosum）LA54a、副肠膜魏斯氏菌（W. paramesenteroides）LA7a和嗜冷明串珠菌（Lc. gelidum）UAL 187-22产生了所有三种细菌素，而副肠膜魏斯氏菌OX和肉明串珠菌TA11a仅产生Leucocin A和B型细菌素。
