Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China; School of Biochemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
Biosens Bioelectron. 2016 Nov 15;85:381-386. doi: 10.1016/j.bios.2016.05.030. Epub 2016 May 10.
A voltammetric sensor for melamine (MEL) was prepared from molecularly imprinted nanohybrids (MINBs). A dopamine modified poly-γ-glutamic acid copolymer (γ-PGA-DA) and MEL were self-assembled into MEL/γ-PGA-DA nanoparticles (NPs) in aqueous solution via weak interactions, followed by adding an aqueous AgNO3 solution into the mixture. The Ag(+) was adsorbed in the MEL/γ-PGA-DA NPs and spontaneously reduced to Ag NPs by the dopamine moieties of γ-PGA-DA, forming Ag/MEL/γ-PGA-DA MINBs, which were then cast on a gold electrode to form a MINBs film. The MEL was removed by electrolysis via catalysis of Ag NPs at a constant potential of 1.4V in phosphate buffer saline solution, to obtain a voltammetric sensor for MEL. The sensor responded linearly to MEL in the concentration range of 5×10(-18) to 5×10(-7)molL(-1). Compared to other published molecularly imprinted polymer sensors for sensing MEL, the prepared MINBs sensor had much wider detection range with lower detection limit.
一种用于三聚氰胺（MEL）的伏安传感器是由分子印迹纳米杂化材料（MINBs）制备的。多巴胺修饰的聚-γ-谷氨酸共聚物（γ-PGA-DA）和 MEL 在水溶液中通过弱相互作用自组装成 MEL/γ-PGA-DA 纳米颗粒（NPs），然后将 AgNO3 水溶液加入混合物中。Ag(+)被吸附在 MEL/γ-PGA-DA NPs 中，并通过 γ-PGA-DA 的多巴胺部分自发还原为 Ag NPs，形成 Ag/MEL/γ-PGA-DA MINBs，然后将其涂覆在金电极上形成 MINBs 膜。在磷酸盐缓冲盐溶液中，通过 Ag NPs 的催化，在 1.4V 的恒电位下电解去除 MEL，从而获得用于 MEL 的伏安传感器。该传感器对 MEL 的响应在 5×10(-18)至 5×10(-7)molL(-1)的浓度范围内呈线性。与其他用于检测 MEL 的已发表的分子印迹聚合物传感器相比，制备的 MINBs 传感器具有更宽的检测范围和更低的检测限。
