Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, 14040-904, São Paulo, SP, Brazil.
Clin Oral Implants Res. 2017 Jul;28(7):e68-e75. doi: 10.1111/clr.12876. Epub 2016 May 19.
The aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant-abutment interface in two different implant connections, external hexagon and tri-channel internal connection, both with conventional flat-head or experimental conical-head abutment screws.
Forty-eight two-part implants with external hexagon (EH; n = 24) or tri-channel internal (TI; n = 24) connections were investigated. Abutments were attached to implants with conventional flat-head or experimental conical-head screws. After saliva incubation, Checkerboard DNA-DNA hybridization was used to identify and quantify up to 38 bacterial colonizing the internal parts of the implants. Kruskal-Wallis test followed by Bonferroni's post-tests for multiple comparisons was used for statistical analysis.
Twenty-four of thirty-eight species, including putative periodontal pathogens, were found colonizing the inner surfaces of both EH and TI implants. Peptostreptococcus anaerobios (P = 0.003), Prevotella melaninogenica (P < 0.0001), and Candida dubliniensis (P < 0.0001) presented significant differences between different groups. Means of total microbial count (×10 , ±SD) for each group were recorded as follows: G1 (0.27 ± 2.04), G2 (0 ± 0), G3 (1.81 ± 7.50), and G4 (0.35 ± 1.81).
Differences in the geometry of implant connections and abutment screws have impacted the microbial leakage through the implant-abutment interface. Implants attached with experimental conical-head abutment screws showed lower counts of microorganisms when compared with conventional flat-head screws.
本体外研究旨在识别和量化通过两种不同种植体连接（外六边连接和三通道内部连接）穿过种植体-基台界面的人唾液中的 38 种微生物，两种连接均采用常规平头或实验性锥形头基台螺丝。
研究了 48 个带有外六边（EH；n=24）或三通道内部（TI；n=24）连接的两部分种植体。将基台用常规平头或实验性锥形头螺丝固定在种植体上。唾液孵育后，采用斑点杂交 DNA-DNA 技术识别和量化定植在种植体内部的多达 38 种细菌。使用 Kruskal-Wallis 检验 followed by Bonferroni 的 post-tests for multiple comparisons 进行统计学分析。
在 EH 和 TI 种植体的内表面发现了包括潜在牙周病原菌在内的 38 种细菌中的 24 种。厌氧消化链球菌（P=0.003）、黑色素普雷沃菌（P<0.0001）和杜氏念珠菌（P<0.0001）在不同组之间存在显著差异。每组的总微生物计数（×10 ，±SD）平均值记录如下：G1（0.27±2.04）、G2（0±0）、G3（1.81±7.50）和 G4（0.35±1.81）。
种植体连接和基台螺丝的几何形状差异影响了种植体-基台界面的微生物渗漏。与常规平头螺丝相比，用实验性锥形头基台螺丝固定的种植体显示出较低的微生物计数。
