Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Clin Oral Implants Res. 2012 Oct;23(10):1168-72. doi: 10.1111/j.1600-0501.2011.02280.x. Epub 2011 Sep 8.
Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant-abutment interface, under non-loading conditions, using either DNA Checkerboard or culture method.
Thirty dental implants with hexagonal platforms were connected to pre-machined abutments according to the manufacturers' specifications. The assemblies were individually incubated in human saliva under anaerobic conditions for 7 days at 37°C. Afterward, contents from the inner parts of the implants were collected and evaluated with either DNA Checkerboard (s = 15) or culture (n = 15). Subsequently, identification and quantitation of bacterial species from saliva and implants were carried out for the group evaluated with the DNA Checkerboard method.
Both DNA Checkerboard and culture showed positive signals of bacterial leakage in 6 of the 15 evaluated samples. Capnocytophaga gingivalis and Streptococcus mutans were the most frequently detected species harboring the internal surface of the implants followed by Veillonella parvula.
Occurrence of bacterial leakage along the implant-abutment interface is comparably detected with both DNA Checkerboard hybridization and conventional culture methods.
由于连接失败，细菌物种已被发现存在于牙种植体的内表面。本研究旨在比较在非加载条件下，使用 DNA checkerboard 或培养方法，从人唾液中检测细菌通过种植体-基台界面泄漏的频率。
将 30 个具有六方平台的牙种植体按照制造商的规格连接到预加工的基台上。将组件分别在 37°C 下的厌氧条件下在人唾液中孵育 7 天。之后，从种植体内部收集内容物，并使用 DNA checkerboard（s=15）或培养法（n=15）进行评估。随后，对使用 DNA checkerboard 方法评估的组进行唾液和种植体中细菌种类的鉴定和定量。
DNA checkerboard 和培养法均在 15 个评估样本中的 6 个样本中显示出细菌泄漏的阳性信号。牙龈二氧化碳嗜纤维菌和变形链球菌是最常检测到的存在于种植体内部表面的物种，其次是小韦荣球菌。
在种植体-基台界面处发生细菌泄漏的情况，使用 DNA checkerboard 杂交和传统培养方法均可检测到。
