Bouvier M, Brown G R
McGill University, Department of Chemistry, Montreal, Canada.
Biochim Biophys Acta. 1989 May 31;991(2):303-9. doi: 10.1016/0304-4165(89)90120-7.
Binding of bilirubin by the alpha-helix conformation of poly(L-lysine) in water induces optical activity. The bisignate circular dichroism spectrum exhibits exciton bands centred at 444 nm, negative, and at 525 nm, positive. The magnitude of the induced circular dichroism depends on the concentration of total bilirubin and total lysine residues, the molar ratio of total lysine residues-to-total bilirubin molecules, the pH and the degree of polymerization of poly(L-lysine). Although bilirubin binds to the random coil conformation of poly(L-lysine), as evidence by the absorption spectrum, the complex is optically inactive. The results suggest that bilirubin binds to the poly(L-lysine) in the form of dimers and oligomers.
胆红素与水中聚（L-赖氨酸）的α-螺旋构象结合会诱导光学活性。双符号圆二色光谱显示出以444nm（负）和525nm（正）为中心的激子带。诱导圆二色性的大小取决于总胆红素和总赖氨酸残基的浓度、总赖氨酸残基与总胆红素分子的摩尔比、pH值以及聚（L-赖氨酸）的聚合度。尽管如吸收光谱所证明的，胆红素与聚（L-赖氨酸）的无规卷曲构象结合，但该复合物没有光学活性。结果表明，胆红素以二聚体和寡聚体的形式与聚（L-赖氨酸）结合。
