Rasti Zarnegar, Nasiri Mahboobeh, Kohan Leila
Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.; Young Researchers and Elite Club, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.
Int J Reprod Biomed. 2016 Feb;14(2):103-8.
Recurrent pregnancy loss (RPL) is defined as two or more miscarriages before the 20(th) week of gestation and its etiology is unknown in 50% of the cases. Interleukin 6 is an immune mediator, plays a regulatory role in embryo implantation and placental development.
The purpose was to assess the association between IL-6 -634C/G polymorphism and, susceptibility to idiopathic RPL for the first time in Iran.
In total 121 women with RPL and 121 healthy women as control group were enrolled in this case-control study. This study was performed from August 2013 to October 2014 in the Molecular Genetics Laboratory of Arsanjan University. Candidate polymorphism was evaluated by PCR-RFLP method on extracted genomic DNA. Data was analyzed using the statistical SPSS package.
Our results showed an increased risk of RPL in patients with GG + GC genotype (OR=5.1, 95%CI: 1.04-25.3, p=0.04) in comparison to CC genotype. The frequency of mutant allele G in patients and controls was 0.75 and 0.66 respectively. The mutant allele G predisposes women to miscarriage 1.5 times greater than controls (OR=1.5, 95%CI: 1.03-2.27, p=0.036). The mean number of live births in RPL women (1.3±2.3) was significantly lower compared to control women (4.8±2.3).
This study indicated that the promoter polymorphism (-634C/G) of the IL-6 gene has likely influence on individual susceptibility to RPL.
复发性流产（RPL）定义为妊娠20周前发生两次或两次以上流产，50%的病例病因不明。白细胞介素6是一种免疫介质，在胚胎着床和胎盘发育中起调节作用。
首次在伊朗评估白细胞介素6基因-634C/G多态性与特发性复发性流产易感性之间的关联。
本病例对照研究共纳入121例复发性流产女性和121例健康女性作为对照组。本研究于2013年8月至2014年10月在阿尔桑詹大学分子遗传学实验室进行。采用PCR-RFLP方法对提取的基因组DNA进行候选多态性评估。使用统计软件SPSS对数据进行分析。
我们的结果显示，与CC基因型相比，GG+GC基因型患者发生复发性流产的风险增加（OR=5.1，95%CI：1.04-25.3，p=0.04）。患者和对照组中突变等位基因G的频率分别为0.75和0.66。突变等位基因G使女性流产的易感性比对照组高1.5倍（OR=1.5，95%CI：1.03-2.27，p=0.036）。复发性流产女性的平均活产数（1.3±2.3）显著低于对照女性（4.8±2.3）。
本研究表明，白细胞介素6基因启动子多态性（-634C/G）可能影响个体对复发性流产的易感性。
