Di Bello C, Gozzini L, Tonellato M, Corradini M G, D'Auria G, Paolillo L, Trivellone E
Biopolymers. 1989 Jan;28(1):421-40. doi: 10.1002/bip.360280139.
The proton nmr characterization of bombesin (BBS) and of two peptide fragments corresponding to the (1-6) and (6-14) sequences has been carried out at 500 MHz in dimethyl sulfoxide (DMSO-d6) using two-dimensional (2D) homo and 1H-13C heterocorrelated techniques. All resonances in the nmr spectra have been assigned and several coupling constants have been measured. The backbone J alpha CH-NH coupling constants are quite similar and around 7.8-8.2 Hz, pointing to an unfolded structure in DMSO-d6. The possibility of secondary structures in highly viscous mixtures of DMSO-d6-water was investigated. The existence of sequential nuclear Overhauser enhancement (NOE) effects in the C-terminal nonapeptide section may indicate a preferential site for secondary structuring.
已在500兆赫下于二甲亚砜（DMSO-d6）中使用二维（2D）同核和1H-13C异核相关技术对蛙皮素（BBS）以及对应于（1-6）和（6-14）序列的两个肽片段进行了质子核磁共振表征。核磁共振谱中的所有共振峰均已归属，并且测量了几个耦合常数。主链JαCH-NH耦合常数相当相似，约为7.8 - 8.2赫兹，表明在DMSO-d6中为无规结构。研究了DMSO-d6 - 水的高粘性混合物中二级结构的可能性。C末端九肽部分中连续核Overhauser效应（NOE）的存在可能表明二级结构形成的优先位点。
