Kuberger M B, Sviridov D D, Izachik N A, Nazhimov V P, Izachik Iu A
Biull Eksp Biol Med. 1989 Apr;107(4):503-5.
The interest in the studies of the intestines using the method of tissue organ culture has considerably grown in recent years. It can be explained by the great possibilities of obtaining unique data about the state of intestines in normal and pathological condition, e.g. malabsorption syndrome. The paper deals with the method modified by the authors to obtain long-living (24 hours) intestinal tissue organ culture. The investigations used bioptic sections which were obtained by jejunoscopy with spot biopsy of children suffering from intestinal malabsorption. The viability of the explants was proved by histological and histochemical tests. The promise held by the methods is emphasized.
近年来，利用组织器官培养方法对肠道进行研究的兴趣显著增加。这可以通过在正常和病理状态下（例如吸收不良综合征）获取有关肠道状态的独特数据的巨大可能性来解释。本文论述了作者改良的用于获得存活24小时的肠道组织器官培养的方法。研究使用了通过空肠镜检查获取的活检切片，并对患有肠道吸收不良的儿童进行了定点活检。通过组织学和组织化学测试证明了外植体的活力。强调了这些方法的前景。
