Tomsová Z, Jůzová Z
Cas Lek Cesk. 1989 Jan 6;128(2):59-61.
Estimation of neopterin in urine has become part of examination in phenylketonuria, malignities, immunodeficiency incl. AIDS and HIV infection. The authors describe a simple chromatographic method for estimation of neopterin which does not call for highly efficient liquid chromatography nor imported kits. The di- and tetrahydro- forms of neopterin are oxidized with MnO2 and stable neopterin is obtained. The specimen is purified on a Dowex 50WX4 (Fluka) column in a H+ cycle and on PRESEPC18 (TESSEK) columns. Then thin-layer chromatography on Merck Kieselgel 60F254 plates is used in a system of ethyl acetate--isopropanol--1 N NH4OH (35:45:25). Fluorescence is assessed on a densitometer (Shimadzu CS-920 at 367.5 nm). The RF of neopterin is 0.214. The fluorescence is linear within the range of 5-200 ng. The method is sufficiently sensitive also for the estimation of normal neopterin excretion. The authors submit the results of estimations in controls, immunopathies and AIDS.
尿液中新蝶呤的测定已成为苯丙酮尿症、恶性肿瘤、免疫缺陷（包括艾滋病和HIV感染）检查的一部分。作者描述了一种简单的色谱法来测定新蝶呤，该方法既不需要高效液相色谱，也不需要进口试剂盒。新蝶呤的二氢和四氢形式用MnO₂氧化，得到稳定的新蝶呤。标本在处于H⁺循环的Dowex 50WX4（Fluka）柱和PRESEPC18（TESSEK）柱上进行纯化。然后在默克硅胶60F254板上，于乙酸乙酯 - 异丙醇 - 1N NH₄OH（35:45:25）系统中进行薄层色谱分析。在密度计（岛津CS - 920，367.5nm）上评估荧光。新蝶呤的比移值为0.214。荧光在5 - 200ng范围内呈线性。该方法对正常新蝶呤排泄量的测定也具有足够的灵敏度。作者提交了对照组、免疫病患者和艾滋病患者的测定结果。
