Cheng Yaxin, Liu Dong, Tang Wenqiao
a Laboratory of Fishes , Shanghai Ocean University, Shanghai Key Laboratory of Marine Animal Taxonomy and Evolution, Ministry of Education , Shanghai , People's Republic of China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Mar;28(2):248-249. doi: 10.3109/19401736.2015.1115863. Epub 2016 May 23.
The complete sequence of the mitochondrial DNA (mtDNA) of the East Asian finless porpoise (Neophocaena asiaeorientails sunamrei) has been completed and annotated in this study. The circular genome is 16 385 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 2 non-coding areas. Ten protein-coding genes use ATG as the initiation codon, except that ND2 started with ATT, and ND3 and ND5 started with ATA. Canonical TAA and TAG termination codons are found in seven protein-coding genes, the remaining four (ND4, ND2, ND3, and COIII) have incomplete termination codons (T or TA), in addition, the COI ends with AGG, and the Cyt b ends with AGA. On the neighbor-joining phylogenetic tree, these results show that the relationship of Phocoenidae is closer with Delphinidae.
本研究完成了东亚江豚(Neophocaena asiaeorientails sunamrei)线粒体DNA(mtDNA)的全序列测定及注释。该环状基因组长度为16385bp,由13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和2个非编码区组成。除ND2起始密码子为ATT,ND3和ND5起始密码子为ATA外,10个蛋白质编码基因使用ATG作为起始密码子。7个蛋白质编码基因中发现了典型的TAA和TAG终止密码子,其余4个(ND4、ND2、ND3和COIII)具有不完全终止密码子(T或TA),此外,COI以AGG结尾,Cyt b以AGA结尾。在邻接法系统发育树上,这些结果表明鼠海豚科与海豚科的关系更为密切。