DNA 在糖酵解溶液中的世界。

The world of DNA in glycol solution.

机构信息

Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK.

出版信息

Nat Rev Mol Cell Biol. 2016 May 23;17(6):335-6. doi: 10.1038/nrm.2016.66.

DOI:10.1038/nrm.2016.66

Abstract

The properties of high-molecular-weight DNA are usually investigated in neutral aqueous solutions. Strong acids and strong alkaline solutions are obviously unsuitable, as are corrosive solvents, and DNA is insoluble in most organic solvents; precipitation of DNA from aqueous solution with ethanol or isopropanol is therefore frequently used as a purification step. An exception is the organic solvent glycol (ethylene glycol, 1,2-ethanediol, dihydroxyethane, HOCH2CH2OH) and the similar solvent glycerol. Double-stranded DNA remains soluble in salt-containing glycol, although it precipitates in polyethylene glycol. (DNA also remains soluble in formamide, but the double-helical structure of DNA is much less stable in this solvent than in glycol.) However, DNA in glycol has been little investigated during the last half-century.

摘要

高分子量 DNA 的性质通常在中性水溶液中进行研究。强酸和强碱性溶液显然不合适，腐蚀性溶剂也不合适，而且大多数有机溶剂中 DNA 不溶；因此，通常使用乙醇或异丙醇从水溶液中沉淀 DNA 作为纯化步骤。一个例外是有机溶剂二醇（乙二醇、1,2-乙二醇、二羟乙烷、HOCH2CH2OH）和类似的溶剂甘油。含有盐的二醇溶液中的双链 DNA 仍然是可溶的，尽管它会在聚乙二醇中沉淀。（DNA 在甲酰胺中也仍然是可溶的，但与在二醇中相比，DNA 的双螺旋结构在这种溶剂中的稳定性要差得多。）然而，在过去的半个世纪中，对二醇中的 DNA 研究甚少。