Shurafa M, Kithier K
Medical Director, Adult Hemophilia Treatment Center, Detroit, Michigan, and Division of Hematology-Oncology, Henry Ford Hospital, 2799 W. Grand Blvd, Detroit, MI 48202, USA.Associate Professor, Department of Pathology, Wayne State University, Detroit, Michigan, USA.
Haemophilia. 1995 Jul;1(3):175-7. doi: 10.1111/j.1365-2516.1995.tb00063.x.
We tested for antibodies against factor VIII by using monoclonal antibody-purified factor VIII preparation as a source of antigen. The factor VIII was adsorbed on nitrocellulose membranes and stored in a refrigerator until later use. Plasma or serum was incubated with the factor VIII containing strip and the antibody was detected by another incubation with peroxidase-labelled antihuman immunoglobulin antibodies. The test was efficient in detecting antibodies in haemophilic and normal subjects with acquired inhibitors to factor VIII. It also detected antibodies to the factor VIII protein in a haemophilic subject with no evidence of inhibitor. The technique is simple, readily applicable, and serves as a useful screening tool for detecting factor VIII antibodies. The stability of the antigen-containing strips in a refrigerator is a practical advantage with potential commercial application.
我们使用单克隆抗体纯化的因子VIII制剂作为抗原来源，检测针对因子VIII的抗体。将因子VIII吸附在硝酸纤维素膜上，储存在冰箱中以备后用。将血浆或血清与含因子VIII的试纸条一起孵育，然后通过与过氧化物酶标记的抗人免疫球蛋白抗体再次孵育来检测抗体。该检测方法在检测血友病患者和获得性因子VIII抑制剂的正常受试者中的抗体方面很有效。它还在没有抑制剂证据的血友病患者中检测到了针对因子VIII蛋白的抗体。该技术简单，易于应用，是检测因子VIII抗体的有用筛选工具。含抗原试纸条在冰箱中的稳定性是一个具有潜在商业应用价值的实际优势。
