Buss F, Jockusch B M
Developmental Biology Unit, University of Bielefeld, FRG.
FEBS Lett. 1989 May 22;249(1):31-4. doi: 10.1016/0014-5793(89)80009-2.
Expression of profilin and profilin:actin ratios in vertebrates were determined with polyclonal antibodies against profilin and actin. Profilin was detected in a wide variety of bovine tissues and was enriched in smooth muscle of bovine, porcine and avian origin. The protein was purified from pig stomach muscle tissue. Smooth muscle profilin was found to be more effective in inhibiting the polymerization of skeletal muscle actin than thymus profilin purified by the same method.
使用抗肌动蛋白结合蛋白和肌动蛋白的多克隆抗体测定了脊椎动物中肌动蛋白结合蛋白的表达及肌动蛋白结合蛋白与肌动蛋白的比例。在多种牛组织中检测到了肌动蛋白结合蛋白，并且在源自牛、猪和禽类的平滑肌中含量丰富。该蛋白从猪胃肌肉组织中纯化得到。发现平滑肌肌动蛋白结合蛋白在抑制骨骼肌肌动蛋白聚合方面比用相同方法纯化的胸腺肌动蛋白结合蛋白更有效。
