Jordanova Maja, Rebok Katerina, Malhão Fernanda, Rocha Maria J, Rocha Eduardo
Laboratory of Histology and Embryology, Institute of Biology, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Mail, Republic of Macedonia.
Laboratory of Histology and Embryology, Department of Microscopy, Institute of Biomedical Sciences Abel Salazar-ICBAS, University of Porto-U.Porto, Porto, Portugal.
Microsc Res Tech. 2016 Aug;79(8):700-6. doi: 10.1002/jemt.22687. Epub 2016 May 25.
This study on wild female Ohrid trout was primarily designed to provide a general overview of the breeding cycle influence upon selected aspects of hepatocytes. According with a semiquantitatively evaluation, some of these cell's structural compartments change during the breeding cycle. Structural modifications were disclosed in the relative occurrence of lipid, glycogen, and RER content during breeding cycle. The relative amount of lipid deposits in the hepatocytes was much greater in previtellogenesis, and decreased postspawning. So, while the seasonal changes in RER were positively related with the ovary maturation status, those of the lipid droplets followed an opposite trend. The hepatocytic glycogen occurred rarely, mainly in late-vitellogenesis and spawning, suggesting that in this species such kind of energy storage is comparatively unimportant. Lipid accumulation and later usage is, probably, the relevant biochemical pathway for Ohrid trout in the wild. While glycogen and RER contents were positively correlated with the gonadosomatic index, lipids were negatively correlated. Additionally, glycogen inclusions were positively correlated with the plasma estradiol levels. When comparing seasonal patterns from wild Ohrid trout with those from well-studied rainbow and brown trout (specimens studied were from aquaculture), there are contradicting results as to lipid and glycogen reserves, and also as to RER loads. The differences among the mentioned trout can result from intrinsic interspecies differences or may be associated with natural feeding conditions versus feeding with commercially prepared diets, or other factors. This study offers new data useful as standard to access liver pathology in wild and aquacultured Ohrid trout. Microsc. Res. Tech. 79:700-706, 2016. © 2016 Wiley Periodicals, Inc.
这项关于野生雌性奥赫里德鳟鱼的研究主要旨在全面概述繁殖周期对肝细胞选定方面的影响。根据半定量评估，这些细胞的一些结构区室在繁殖周期中会发生变化。在繁殖周期中，脂质、糖原和粗面内质网（RER）含量的相对发生率出现了结构改变。肝细胞中脂质沉积的相对量在卵黄生成前期要多得多，而在产卵后减少。因此，虽然RER的季节性变化与卵巢成熟状态呈正相关，但脂滴的变化趋势则相反。肝细胞糖原很少出现，主要在卵黄生成后期和产卵期，这表明在该物种中，这种能量储存方式相对不太重要。脂质积累及其后的利用可能是野生奥赫里德鳟鱼的相关生化途径。虽然糖原和RER含量与性腺指数呈正相关，但脂质则呈负相关。此外，糖原内含物与血浆雌二醇水平呈正相关。当将野生奥赫里德鳟鱼的季节性模式与经过充分研究的虹鳟和褐鳟（研究的样本来自水产养殖）的模式进行比较时，在脂质和糖原储备以及RER负荷方面存在相互矛盾的结果。上述鳟鱼之间的差异可能源于物种内在差异，也可能与自然摄食条件与商业配制饲料喂养的差异或其他因素有关。这项研究提供了新的数据，可作为评估野生和养殖奥赫里德鳟鱼肝脏病理学的标准。《显微镜研究与技术》79:700 - 706, 2016年。© 2016威利期刊公司
