Sacks P G, Oke V, Vasey T, Lotan R
Department of Head and Neck Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Head Neck. 1989 May-Jun;11(3):219-25. doi: 10.1002/hed.2880110305.
The effects of retinoic acid (RA) on multicellular tumor spheroids (MTS) derived from a head and neck squamous carcinoma cell line, MDA 886Ln, were studied. Growth of MTSs was shown to be inhibited by 10(-5) to 10(-7) M RA; dose response studies demonstrated that by 10(-10) M RA, MTS growth was inhibited by less than 20%. MTSs treated with RA for 10 days had a decreased labeling index (15% compared with 23% for controls). Histologic studies at 10 days showed both an alteration in tissue-like architecture and an inhibition of squamous differentiation in RA-treated spheroids. Morphologic inhibition of differentiation was confirmed by immunohistochemical staining for involucrin. Histologic sections were also probed with a series of biotinylated lectins to search for RA-induced changes in glycosylation. Changes in staining occurred with two lectins, soybean agglutinin and peanut agglutinin. This study showed that RA induced perturbations in biological processes such as growth and differentiation in a new model system for squamous carcinomas of the head and neck, MDA 886Ln MTS.
研究了维甲酸（RA）对源自头颈鳞状癌细胞系MDA 886Ln的多细胞肿瘤球体（MTS）的影响。结果显示，10^(-5)至10^(-7) M的RA可抑制MTS的生长；剂量反应研究表明，10^(-10) M的RA对MTS生长的抑制率低于20%。用RA处理10天的MTS标记指数降低（与对照组的23%相比为15%）。10天时的组织学研究显示，经RA处理的球体在组织样结构上发生改变，且鳞状分化受到抑制。通过对兜甲蛋白进行免疫组织化学染色证实了分化的形态学抑制。还使用一系列生物素化凝集素对组织学切片进行检测，以寻找RA诱导的糖基化变化。两种凝集素，即大豆凝集素和花生凝集素，出现了染色变化。本研究表明，在头颈鳞状癌的新模型系统MDA 886Ln MTS中，RA诱导了生长和分化等生物过程的紊乱。
