Mathews L S, Enberg B, Norstedt G
Center for Biotechnology, Huddinge University Hospital, Sweden.
J Biol Chem. 1989 Jun 15;264(17):9905-10.
A cDNA encoding the growth hormone (GH) receptor was cloned from rat liver. Both the nucleotide and translated amino acid sequence share greater than 70% similarity with the GH receptors from rabbit and human. An RNA probe was generated from this sequence for use in a solution hybridization assay to quantitate GH receptor mRNA expression in rat tissues. Expression was detected in 9/12 tissues examined, with the highest levels observed in the liver. Expression in liver, kidney, heart and muscle was developmentally regulated, being low at birth and rising to adult levels in 5 weeks. No difference was observed between hepatic expression in males and females, although livers from pregnant rats had elevated levels. Hypophysectomy and GH treatment did not affect hepatic GH receptor mRNA levels.
从大鼠肝脏中克隆出编码生长激素（GH）受体的cDNA。其核苷酸序列和翻译后的氨基酸序列与兔和人的GH受体均有超过70%的相似性。利用该序列制备RNA探针，用于溶液杂交试验以定量大鼠组织中GH受体mRNA的表达。在所检测的12种组织中有9种检测到了表达，其中肝脏中的表达水平最高。肝脏、肾脏、心脏和肌肉中的表达受发育调控，出生时表达水平较低，5周时升至成年水平。雄性和雌性大鼠肝脏中的表达没有差异，不过怀孕大鼠肝脏中的表达水平有所升高。垂体切除和GH处理不影响肝脏中GH受体mRNA的水平。
