一种利用细胞质定位荧光探针评估细胞多核化的高分辨率方法。

A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes.

机构信息

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.

College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.

出版信息

Analyst. 2016 Jun 20;141(13):4010-3. doi: 10.1039/c6an00613b.

DOI:10.1039/c6an00613b

PMID:27229293

Abstract

Cell multinucleation is closely related to chromosomal instability. We report a simple, convenient method to assess cell multinucleation with cytoplasm-localized fluorescent probes (CLFP) which is superior to conventional nuclear staining methods. The CLFP method provides high-resolution images that enable the accurate calculation of the number of nuclear fragments.

摘要

细胞多核化与染色体不稳定性密切相关。我们报告了一种简单、方便的评估细胞质定位荧光探针 (CLFP) 细胞多核化的方法，优于传统的核染色方法。CLFP 方法提供高分辨率图像，能够准确计算核片段的数量。