Huang F L, Yoshida Y, Nakabayashi H, Friedman D P, Ungerleider L G, Young W S, Huang K P
National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
J Cell Biochem. 1989 Apr;39(4):401-10. doi: 10.1002/jcb.240390406.
Previously using PKC isozyme-specific antibodies for immunoblot analysis, we demonstrated the heterogeneous distribution of PKC isozymes in various regions of monkey and rat brains and that type I PKC was most abundant in cerebellum, hippocampus, amygdala, and cerebral cortex (Huang et al.: J Biol Chem 262:15714-15720, 1987). Using these antibodies, we have also demonstrated that type I, II, and III PKC are products of PKC genes gamma, beta, and alpha, respectively (Huang et al.: Biochem Biophys Res Commun 149:946-952, 1987). By immunocytochemical analysis, type I PKC-specific antibody showed strong reactivity in various types of neuron in hippocampal formation, amygdala, cerebellum, and neocortex. In hippocampal formation, granule cells of dentate gyrus and pyramidal cells of hippocampus were heavily stained. By immunoblot analysis, relative levels of PKC isozymes in several areas of monkey cerebral cortex involved in the visual information processing and storage were determined. Both type II and III PKCs appeared to be evenly distributed and at moderate levels, type I PKC formed a gradient of increasing concentration rostral along the cerebral cortex of occipital to temporal and then to the limbic areas. Neurobehavioral studies have demonstrated that the neocortical and limbic areas of the anterior and medial temporal regions participate more directly than the striate, prestriate, and posterior temporal regions in the storage of visual representations and that both hippocampus and amygdala are important in the memory formation. As type I PKC is present at high levels in hippocampus, amygdala, and anterior temporal lobe, we predict that the type I protein kinase C may participate in the plastic changes important for mnemonic function.
此前，我们使用蛋白激酶C（PKC）同工酶特异性抗体进行免疫印迹分析，证明了PKC同工酶在猴脑和大鼠脑的不同区域中分布不均，其中I型PKC在小脑、海马、杏仁核和大脑皮层中含量最为丰富（黄等人：《生物化学杂志》262:15714 - 15720，1987年）。使用这些抗体，我们还证明了I型、II型和III型PKC分别是PKC基因γ、β和α的产物（黄等人：《生物化学与生物物理研究通讯》149:946 - 952，1987年）。通过免疫细胞化学分析，I型PKC特异性抗体在海马结构、杏仁核、小脑和新皮层的各类神经元中显示出强反应性。在海马结构中，齿状回的颗粒细胞和海马的锥体细胞被重度染色。通过免疫印迹分析，测定了参与视觉信息处理和存储的猴大脑皮层几个区域中PKC同工酶的相对水平。II型和III型PKC似乎分布均匀且水平适中，I型PKC沿枕叶到颞叶再到边缘区域的大脑皮层形成了一个浓度从尾端向头端增加的梯度。神经行为学研究表明，颞叶前部和内侧的新皮层和边缘区域比纹状区、纹状前区和颞叶后部区域更直接地参与视觉表象的存储，并且海马和杏仁核在记忆形成中都很重要。由于I型PKC在海马、杏仁核和颞叶前部含量很高，我们预测I型蛋白激酶C可能参与对记忆功能至关重要的可塑性变化。
