Dauchel M C, Courty J, Mereau A, Barritault D
Laboratoire de Biotechnologie des Cellules Eucaryotes, Université Paris XII, Créteil, France.
J Cell Biochem. 1989 Apr;39(4):411-20. doi: 10.1002/jcb.240390407.
Polycationic molecules were studied either for their ability to displace the binding of basic fibroblast growth factor (bFGF) to high- and low-affinity membrane interaction sites and/or to modulate bFGF-induced proliferation of fibroblasts. Heparin-binding polypeptides, such as polylysine, protamine, histones, and thrombin-displaced [125I]bFGF bound to bovine brain membrane receptors. The most displacing polypeptides were those with the strongest affinity to heparin. Two of these polypeptides, protamine and polylysine, inhibited (at 5 microM) by more than 90% the mitogenic effect induced by bFGF on Chinese hamster lung fibroblast cells (CCL39). At the same dose, no effect was observed with basic proteins that do not bind to heparin, such as cytochrome C and lysozyme. An interesting observation was that protamine at 1 microM potentiated by 1.5-fold the mitogenic activity of bFGF, while it acted as an inhibitor at higher concentration.
对多阳离子分子进行了研究，以考察它们取代碱性成纤维细胞生长因子（bFGF）与高亲和力和低亲和力膜相互作用位点结合的能力，以及/或者调节bFGF诱导的成纤维细胞增殖的能力。肝素结合多肽，如聚赖氨酸、鱼精蛋白、组蛋白，以及凝血酶置换的[125I]bFGF与牛脑膜受体结合。置换能力最强的多肽是那些与肝素亲和力最强的多肽。其中两种多肽，鱼精蛋白和聚赖氨酸，在5微摩尔浓度时可抑制bFGF对中国仓鼠肺成纤维细胞（CCL39）诱导的促有丝分裂效应达90%以上。在相同剂量下，对不与肝素结合的碱性蛋白，如细胞色素C和溶菌酶，未观察到任何作用。一个有趣的发现是，1微摩尔浓度的鱼精蛋白可使bFGF的促有丝分裂活性增强1.5倍，而在较高浓度时它则起抑制剂的作用。
