el-Sayed Y M, Islam S I
Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.
J Clin Pharm Ther. 1989 Apr;14(2):127-34. doi: 10.1111/j.1365-2710.1989.tb00231.x.
Theophylline in serum was measured by fluorescence polarization immunoassay (FPIA) and by high-performance liquid chromatography (HPLC). Within-run precision studies using control samples in the subtherapeutic, therapeutic and toxic concentrations, resulted in coefficients of variation in the range of 2.86-3.12% (FPIA) and 2.1-3.66% (HPLC), respectively. Between-run precision ranged from 2.76-6.2% for FPIA and from 2.51-6.0% for HPLC. The mean recovery for three spiked controls was 98.9% for FPIA and 98.8% for HPLC. Comparison of 60 patients' samples, assayed with both methods, indicated an extremely good analytical correlation (r = 0.990). The FPIA method displayed a slight but consistent positive bias in relation to the concentration of theophylline present in patients sera. Caffeine was found to exhibit a positive bias to 13%, over a caffeine concentration range of 10-40 micrograms/ml. The HPLC method offers an advantage for measurements of both caffeine and theophylline simultaneously. The FPIA offers significant advantages in speed of analysis and turnover-time, while maintaining accuracy and precision compared with those of established HPLC procedures.
采用荧光偏振免疫分析法（FPIA）和高效液相色谱法（HPLC）测定血清中的茶碱。使用亚治疗浓度、治疗浓度和中毒浓度的对照样品进行批内精密度研究，结果FPIA的变异系数范围为2.86 - 3.12%，HPLC的变异系数范围为2.1 - 3.66%。FPIA的批间精密度范围为2.76 - 6.2%，HPLC的批间精密度范围为2.51 - 6.0%。三种加标对照品的平均回收率FPIA为98.9%，HPLC为98.8%。用两种方法检测60例患者样本的比较表明，分析相关性非常好（r = 0.990）。FPIA方法相对于患者血清中茶碱的浓度显示出轻微但一致的正偏差。发现在10 - 40微克/毫升的咖啡因浓度范围内，咖啡因存在13%的正偏差。HPLC方法在同时测定咖啡因和茶碱方面具有优势。与既定的HPLC程序相比，FPIA在分析速度和周转时间方面具有显著优势，同时保持了准确性和精密度。
