Kordossi A A, Tzartos S J
Hellenic Pasteur Institute, Athens, Greece.
J Neuroimmunol. 1989 Jun;23(1):35-40. doi: 10.1016/0165-5728(89)90070-2.
About two-thirds of the antibodies to the nicotinic acetylcholine receptor (AChR) in myasthenic patients, and in rats immunized with intact AChR, bind to the main immunogenic region (MIR) on the alpha-subunit. We tested all available anti-MIR monoclonal antibodies (mAbs) by competition experiments for binding on the intact AChR from Torpedo electric organ and human muscle. Practically complete competition between all possible paired combinations of anti-MIR mAbs was found. As a consequence, the MIR must be a very concrete and small region. Furthermore, the location of the MIR relative to some other less immunogenic regions was also determined.
重症肌无力患者以及用完整的烟碱型乙酰胆碱受体（AChR）免疫的大鼠体内，约三分之二的抗AChR抗体与α亚基上的主要免疫原性区域（MIR）结合。我们通过竞争实验，检测了所有可用的抗MIR单克隆抗体（mAb）与电鳐电器官及人类肌肉中的完整AChR的结合情况。结果发现，抗MIR mAb的所有可能配对组合之间几乎完全竞争。因此，MIR必定是一个非常具体且小的区域。此外，还确定了MIR相对于其他一些免疫原性较低区域的位置。
